Crustaceans have been recognized as rich sources of bioactive compounds with valuable nutraceutical and pharmaceutical potentials. Fresh water crab Oziotelphusa senex senex is the abundantly existing crab with unknown health benefits Cellular damage caused by reactive oxygen species has been implicated in several diseases; hence antioxidants have significant importance in human health Antioxidants play an important role as health protecting factor.Scientific evidences suggests that antioxidants reduce the risk for chronic diseases including cancer and heart diseases .So the.the antioxidant activity of the hemolymph from the fresh water Crab Oziotelphusa senex senex was. evaluated .Hemolymph of Oziotelphusa senex senex a fresh water crab, were subjected for its activity using DPPH,ABTS and hydrogen peroxide scavenging assays. In antioxidant assay, the percentage of 2, 2-diphenyl-1-picrylhydrazyl scavenging activity was recorded as 57.5%. ABTS scavenging activity (68%) hydrogen peroxide scavenging (64%).The antioxidant activity of the hemolymph increased in a concentration dependent manner. Hence, the present study revealed that the hemolymph from the fresh water crab Oziotelphusa senex senex can be used as an accessible source of natural antioxidants with consequent health benefits.