International Journal of Pharma and Bio Sciences
 
 
    ISSN 0975-6299
www.ijpbs.net
RESEARCH ARTICLE
Int J Pharm Bio Sci Volume 7, Issue 3, 2016 (July-Sept), Pages:860-865
ISOLATION, SCREENING OF PHYTATE DEGRADING FUNGI (ASPERGILLUS NIGER) AND OPTIMIZATION OF SUBMERGED FERMENTATIVE PRODUCTION OF PHYTASE
CH.M.KUMARI CHITTURI, P. JEEVANA LAKSHMI
DOI:
[Download PDF]
Abstract:

Microbial phytases are having several applications and one of the important application is it can be used as an animal feed supplement, not only to improve phytate digestibility and nutritive value of plant based foods but also reduces the environmental phosphorus pollution. In the present study, 12 fungal strains were isolated from different poultry soil wastes (Tirupati & surrounding areas) and screened for phytase activity by using phytase screening medium (PSM). Among the isolates one strain was found to possess potent phytase producing activity and identified as Aspergillus niger which has been selected for further studies. Phytase production by A.niger was evaluated in submerged fermentation. Effect of different culture media on phytase production was assessed and fermentation parameters were optimized. Physical parameters (temperature, pH), chemical parameters (different carbon and nitrogen sources and mineral salts) Optimization studies were studied. Maximum phytase activity of 465 U/ml was found at 30°C in submerged cultivation. The activity was found to decline above 30°C. The fungus failed to grow above 50°C. Phytase was most active at pH 5 (456 U/ml) with an increase in the pH of the medium enzyme activity declined. Optimization studies indicated that glucose was more suitable carbon source for maximum (478 U/ml) phytase production. Various nitrogen sources were used in optimization process where peptone has exhibited maximum activity of 482U/ml. It was found in our results that all the metal salts (sodium chloride, magnesium sulphate, manganese sulphate and copper sulphate) used in the study had a negative impact on the enzyme production.

Keywords: phytate-degrading enzyme, feed upplement, Aspergillus niger, optimization, submerged fermentation,ni
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy
Pharmaceutical Fields
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Pharmaceutics
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Novel drug delivery system
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Nanotechnology
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Pharmacology
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Pharmacognosy
© Copyright 2009-2015 IJPBS, India. All rights reserved. Specialized online journals by ubijournal. Website by Ubitech Solutions
         Home I Contact I Terms & Conditions