International Journal of Pharma and Bio Sciences
 
 
    ISSN 0975-6299
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RESEARCH ARTICLE
Int J Pharm Bio Sci Volume 7, Issue 3, 2016 (July-Sept), Pages:180-190
ANALYSIS OF GENETIC DIVERSITY IN CONGENERIC SPECIES OF GRASSHOPPERS BY GEL ANALYSIS OF PROTEINS, ALPHA AMYLASES AND GENOMIC DNA (BY RAPD-PCR)
JAYASHREE. H, CHANNAVEERAPPA. H
DOI:
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Abstract:

Genetic variation between the six congeneric species of grasshoppers has been analyzed by SDS-PAGE (Sodium dodecyl sulfate polyacrylamide gel electrophoresis) and RAPD-PCR technique. The study revealed the presence of polymorphism in muscle proteins ,alpha amylases and in genomic DNA between each congeneric species. Each species had different number of proteins and amylase electromorphs indicating as many number of genes. The RAPD –PCR generated large number of polymorphic bands by each marker applied and also revealed large number of genomic duplications, highest duplication in genome and polymorphism in genomic DNA was observed in C.trachypterus and C.oxypterus followed by A.exaltata and A.gigantica in contrast more similarity for DNA recorded in O.abruptus and O.senegalensis. These genetically divergent ,sympatric and congeneric species cooccurred without any habitat specialization.

Keywords: Congeneric Species, Morphology, Sympatric, Genetic Diversity, Polymorphism, Amylase isoform Extra ba
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