The objective of the current study was to evaluate the antioxidant activity of Citrus peel essence by using DPPH radical scavenging activity and β- carotene test Methods The essence of four varieties of Citrus: C. Sinensis, C. paradisi, C. reticulata and C. aurantium were been extracted by cold expression, then the extract was analyzed to determine the chemical composition by Gas Chromatography coupled to Mass spectrophotometry (GC/MS). The antioxidant activity of Citrus essences was assessed by DPPH
and β- carotene test comparatively to BHT and Quercetin. The results obtained demonstrated that the IC 50 of Citrus essences range from : 1,9068 ± 0,0912 μg ml-1 for Citrus reticulata, 6,2086 ± 0,0308 μg ml-1 for Citrus aurantium, 12,7662 ± 0,0736 μg ml-1 for Citrus paradisii, and 19,1567 ± 0,00499 μg ml-1 for Citrus sinensis, which were all lower than the lC50 of the synthetic antioxidants : Quercetin (IC50 15,9938 ± 0,01284 μg ml-1) and Butylhydroxyanisole (BHA) (IC50 21,9938 ± 0,01284μg ml-1). In conclusion it has been suggested that Citrus essences constituent a real source for natural antioxidant that can be used in several domain such as : pharmaceutics , culinary and food engineering.