International Journal of Pharma and Bio Sciences
 
 
    ISSN 0975-6299
www.ijpbs.net
RESEARCH ARTICLE
Int J Pharm Bio Sci Volume 7, Issue 3, 2016 (July-Sept), Pages:355- 360
COMPARATIVE RELIABILITY OF DIFFERENT METHODS OF SERUM CREATININE ESTIMATION IN JAUNDICE PATIENTS FOR MINIMISINGNEGATIVE INTERFERENCE OF BILURIBIN
CHEEMALAVAGUPALLI REKHA, MANCHALA SRESHMITHA
DOI:
[Download PDF]
Abstract:

One hundred and twenty three (123) blood samples from icteric patients, received for serum creatinine estimation were divided into different groups depending on bilirubin concentration -- Group I (1-5 mg/dl), Group II (5 -15 mg/dl), Group III (15-25 mg/dl), Group IV (>25 mg/dl). Twenty (20) blood samples from non-icteric patients within the normal reference range of bilirubin (0.2 - 0.8 mg/dl) were taken as controls, group V. All these samples were estimated for serum creatinine by four methods (1) Jaffe’s kinetic method, (2) pre-incubation with NaOH method, (3) addition of SDS method and (4) enzymatic method (reference method). A significant increase in serum true creatinine was founded by pre-incubation with 200mmol/L NaOH and the addition of SDS method for bilirubin concentration > 15 mg/dl (p < 0.01) and mild to moderate increase for bilirubin concentration 1-15 mg/dl. From this study, it was found that increased concentration of bilirubin interfered in creatinine value measurement except in Jaffe’s kinetic method. 

Keywords: Glomerular Filtration Rate, Isotope Dilution Mass Spectrometry, Serum Bilirubin, Serum Creatinine, J
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy
Pharmaceutical Fields
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Pharmaceutics
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Novel drug delivery system
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Nanotechnology
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Pharmacology
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Pharmacognosy
© Copyright 2009-2015 IJPBS, India. All rights reserved. Specialized online journals by ubijournal. Website by Ubitech Solutions
         Home I Contact I Terms & Conditions