International Journal of Pharma and Bio Sciences
ijpbs.net
editorijpbs@rediffmail.com (or) editorofijpbs@yahoo.com (or) prasmol@rediffmail.com
10.22376/ijpbs.2019.10.1.p1-12
Volume 3 Issue 4
2012(October - December)
A NOVEL RP-HPLC METHOD FOR THE QUANTIFICATION OF NEPAFENAC IN FORMULATION, PLASMA(IN VITRO
A simple, precise and accurate RP-HPLC method was developed and validated for rapid assay of Nepafenac in tablet dosage form. Isocratic elution at a flow rate of 1ml/min was employed on a symmetry Chromosil C18 (250x4.6mm, 5µm in particle size) at ambient temperature. The mobile phase consisted of Methanol: ACN: THF 50:40:10 (V/V). The UV detection wavelength was 238 nm and 20µl sample was injected. The retention time for Nepafenac was 3.26 min. The percentage RSD for precision and accuracy of the method was found to be less than 2%. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of Nepafenac in tablet dosage form, bulk drug and plasma sample.
V. PHANI KUMAR AND Y. SUNANDAMMA
Nepafenac, RP-HPLC, UV detection, recovery, precise, 238 nm, Plasma sample
847-854