International Journal of Pharma and Bio Sciences
ijpbs.net
editorijpbs@rediffmail.com (or) editorofijpbs@yahoo.com (or) prasmol@rediffmail.com
10.22376/ijpbs.2019.10.1.p1-12
Volume 4 Issue 3
2013 (July - September)
RP-HPLC METHOD FOR THE QUANTIFICATION OF GEMCITABINEIN FORMULATIONS
A simple, precise and accurate RP-HPLC method was developed and validated for rapid assay of Gemcitabine in tablet dosage form. Isocratic elution at a flow rate of 1ml/min was employed on a symmetry Chromosil C18 (250x4.6mm, 5µm in particle size) at ambient temperature. The mobile phase consisted of Methanol: Acetonitrile: Water 80:15:5v/v. The UV detector wavelength was fixed at 282nm. In chromatogram the retention of Gemcitabine was 5.02min. The percentage RSD for precision and accuracy of the method was found to be less than 2%. The method was validated as per the ICH guidelines. The method was successfully applied for regular analysis of Gemcitabine in tablet dosage form.
SUBHASHINI.EDLA AND B.SYAMA SUNDHAR
Gemcitabine, RP-HPLC, UV detection, recovery, precise, 282nm
512-518