International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 4 Issue 3
2013 (July - September)
PHENOLIC ACIDS AND FLAVONOIDS IN HYPERICUM PERFORATUM L. HAIRY ROOTS
Investigations have been made to develop an efficient protocol for identification and quantification of phenolic acids and flavonoids in hairy roots (HR) of lessThan i greaterThan Hypericum perforatum lessThan /i greaterThan L. HR were induced from root segments of lessThan i greaterThan in vitro lessThan /i greaterThan grown seedlings from lessThan i greaterThan H. perforatum lessThan /i greaterThan , after co-cultivation with lessThan i greaterThan Agrobacterium rhizogenes lessThan /i greaterThan A4. Transgenic status of HR was confirmed by PCR analysis using lessThan i greaterThan rol lessThan /i greaterThan B specific primers. HR grew rapidly on hormone-free medium and had plagiotropic growth with vigorous production of lateral roots. Phenolic acids and flavonoids in control roots and HR were analyzed using HPLC/DAD/ESI-MS lessThan sup greaterThan n lessThan /sup greaterThan . Quinic acid was the only detectable phenolic acid in HR. Transgenic roots produced flavonol glycosides such as quercetin 6- lessThan i greaterThan C lessThan /i greaterThan -glucoside, rutin and isorhamnetin lessThan i greaterThan O lessThan /i greaterThan -hexoside. Chromatographic analysis of flavonoid aglycones in HR resulted in the identiï¬cation of kaempferol. Transformed roots yielded higher levels of catechin and epicatechin than untransformed roots. lessThan i greaterThan H. perforatum lessThan /i greaterThan HR represent promising experimental system for enhanced production of phenolic compounds.
OLIVER TUSEVSKI AND SONJA GADZOVSKA SIMIC
Hypericum perforatum L., Agrobacterium rhizogenes A4, hairy roots, phenolic acids, flavonols, flavan-3-ols.
737-748