International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 1 Issue 3
2010 (July - September)
Method Development and Validation for the Estimation of Atazanavir in Bulk and Pharmaceutical Dosage Forms and its Stress Degradation Studies using UV-VIS Spectrophotometric Method
The present study describes a simple, accurate, precise and cost effective UV-VIS Spectrophotometric method for the estimation of Atazanavir, an anti-HIV drug, in bulk and pharmaceutical dosage form. The solvent used was methanol and the λmax or the absorption maxima of the drug was found to be 250nm. A linear response was observed in the range of 1050µg/ml with a regression coefficient of 0.999. The method was then validated for different parameters as per the ICH (International Conference for Harmonization) guidelines. This method can be used for the determination of Atazanavir in quality control of formulation without interference of the excipients. Atazanavir sulphate was subjected to stress degradation under different conditions recommended by ICH. The samples so generated were used for degradation studies using the developed method. lessThan br / greaterThan
Suddhasattya Dey,Y. Vikram Reddy,Thirupathi Reddy,Sudhir Kumar Sahoo,P. N. Murthy,Subhasis Mohapatra,S. Subhasis Patro
Atazanavir, HIV, λmax, ICH, UV-VIS spectroscopy.
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