International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 5 Issue 4
2014 (October - December)
A FOURIER TRANSFORM INFRARED (FTIR) SPECTROSCOPIC STUDY ON CELLULAR CHANGES IN THE MARINOCOCCUS LUTEUS SSLB 1 UNDER DIFFERENT SALINITY REGIME
lessThan i greaterThan Marinococcus luteus lessThan /i greaterThan SSLB1, isolated from the Sambhar Salt Lake, Rajasthan, India, could tolerate NaCl upto 25%, (w/v) concentration in a complex medium. The overall structural and compositional changes in the bacterial isolate in the presence of variable concentrations of NaCl (0-25%) was analyzed by Fourier transform infrared (FTIR) spectroscopic techniques. Results on FTIR analysis showed NaCl induced changes in the absorption peaks corresponding to different functional groups in the lessThan i greaterThan M. luteus lessThan /i greaterThan SSLB1. The wavenumber 1537.6 cm lessThan sup greaterThan -1 lessThan /sup greaterThan (Amide- II), 1538.9 and 1544.5 cm lessThan sup greaterThan -1 lessThan /sup greaterThan N-H (symmetric) bending exhibited band shift at lower NaCl concentration, but they disappeared at the higher concentrations. Similarly FTIR bands observed between 1300-1500 cm lessThan sup greaterThan -1 lessThan /sup greaterThan wave numbers, arising predominately from greaterThan CH lessThan sub greaterThan 3 lessThan /sub greaterThan symmetric deformation, COO lessThan sup greaterThan 2- lessThan /sup greaterThan stretching (symmetric) of fatty acids and wavenumber 1400.9 cm lessThan sup greaterThan -1 lessThan /sup greaterThan shifted to higher frequency of wave numbers. These results indicated NaCl induced adaptational and structural changes in lessThan i greaterThan M. luteus lessThan /i greaterThan . as evident from increase or decrease or shift in the characteristic FTIR peaks assigned to different functional groups.
PREM CHANDRA, MANJARI BARSAINYA AND D.P. SINGH
FTIR, Sambhar Salt Lake, Sodium Chloride, Marinococcus luteus SSLB 1
848-854