International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 5 Issue 4
2014 (October - December)
MICROPROPAGATION OF PIMPINELLA CANDOLLEANA WIGHT & ARN. - AN ENDEMIC MEDICINAL HERB FROM SOUTH INDIA
Micropropagation protocol of lessThan i greaterThan Pimpinella candolleana lessThan /i greaterThan an important ethno medicinal herb has been developed for the first time. High frequency plantlet regeneration was achieved from shoot tip explants of lessThan i greaterThan P. candolleana lessThan /i greaterThan . Effect of growth regulators in different concentrations and combination of BAP, KN, NAA, IAA and IBA on lessThan i greaterThan in vitro lessThan /i greaterThan morphogenesis was tried. The highest regeneration response was observed in Murashige and Skoog's Basal Medium (MSBM) fortified with BAP (13.31µM) and NAA (2.69 µM) where 94 % of the cultures responded with an average set number of 7.20 ±. 0.81 per explant in six weeks time. The best elongation response was noticed on MSBM supplemented with BAP 13.31µM + GA lessThan sub greaterThan 3 lessThan /sub greaterThan 1.44 µM where 93% of the shoots attained the average height of 6.80 ± 0.50. Rooting was achieved on half strength MSBM with 9.8 µM IBA. The plantlets with well developed shoot and root system were hardened and acclimatized to the natural habitat with 90% survival frequency.
A. SRILAKSHMI AND M.C. GAYATRI
Apiaceae, Pimpinella candolleana, micropropogation and MSBM
253-259