International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 5 Issue 4
2014 (October - December)
PRODUCTION AND PURIFICATION OF A THERMOSTABLE LIPASE FROM A HYPERACTIVE BACTERIAL ISOLATE BREVIBACILLUS SP. FROM TAPTAPANI HOT SPRING, ODISHA
Thermostable lipase producing lessThan i greaterThan Brevibacillus sp. lessThan /i greaterThan was isolated from Taptapani hot water spring of Ganjam, Odisha, India. Maximum lipase production by lessThan i greaterThan Brevibacillus sp. lessThan /i greaterThan was obtained at pH 10 when grown under shaking condition at 60 °C for 30 hrs. To enhance the production of lipase, nutritional factors were optimized and it was observed that maximum lipase production was obtained in the presence of ammonium ferric sulfate as the best inorganic nitrogen source. Out of various natural and synthetic triglycerides used, olive oil was found as the most suited substrate for the production of extracellular lipase. In addition to this the carbon source maltose added in this study significantly increases the lipase production. The 2.83 fold purification of the lipase enzyme from this strain with 70% recovery achieved by ammonium sulphate saturation and Phenyl Sepharose column chromatography (specific activity; 2.1U/mg of protein with a molecular weight of 70 kDa) as a single step purification process. It is the first report that a genus lessThan i greaterThan Brevibacillus lessThan /i greaterThan produces large lipase with a size of approximately 70 kDa.
ASHOK KUMAR PANIGRAHI, AMRITA KUMARI PANDA AND S.P.S. BISHT
Brevibacillus sp., thermostable lipase, Phenyl Sepharose.
320-327