International Journal of Pharma and Bio Sciences
ijpbs.net
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10.22376/ijpbs.2019.10.1.p1-12
Volume 6 Issue 1
2015 (January - March)
ISOLATION, IDENTIFICATION, PARTIAL PURIFICATION, OPTIMIZATION AND CHARACTERIZATION OF ALKALINE PROTEASE FROM E. coli
In the present study lessThan i greaterThan E. coli lessThan /i greaterThan was used for the production of alkaline protease in eight different fermentation media. This bacterium was isolated from soil sample and screened for protease production. The enzyme production from lessThan i greaterThan E. coli lessThan /i greaterThan was estimated using casein as substrate and its molecular weight was determined by SDS-PAGE. The enzyme activity was also studied at different parameters like pH, temperature, carbon source, nitrogen source, metal ions concentration and effect of inhibitors.
SHRUTI SHUKLA AND O. P. VERMA
E.coli, alkaline protease, SDS-PSGE, submerged fermentation, optimization.
1081-1088