International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 6 Issue 1
2015 (January - March)
IN VITRO MULTIPLICATION OF IMPORTANT HORTICULTURE PLANTCITRUS RETICULATA (BLANCO.)
Present research work was initiated role of BAP (benzyl amino purine),IAA and 2, 4-Don growth of callus and formation of multiple shoots by using shoot tip, leaf, and nodal explant. lessThan i greaterThan In vitro lessThan /i greaterThan shoot tips, leaf and nodal segment explants were inoculated on MS media supplemented with different concentrations of BAP and IAA (Indole-3- acetic acid) to produce multiple shoots as well as Calli. Shoot tip explants inoculated on MS medium supplemented of2.6 mg/LBAP combination with 0.4 mg/LIAA produce the highest number of shoots per explant was observed. The average numbers of shoot per explants were observed 2.5 mg/L and 0.2 mg/L IBA and IAA respectively. However, nodal segments of lessThan i greaterThan C. reticulate lessThan /i greaterThan inoculated on MS media containing 2 mg/LBAP and 1.5 mg/L IAA responsible for induction of callus was also observed.
VISHWANATH WAGHMARE AND NARAYAN PANDHURE
In vitro, Citrus reticulata, callus induction, Shoot Regeneration.
1275-1280