International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 7 Issue 2
2016 (April - June)
DEVELOPMENT OF A NEW HIGH PERFORMANCE LIQUID HROMATOGRAPHY METHOD FOR VITAMIN A &Β-CAROTENE IN HEALTHY BLOOD SERUM
To develop a new rapid and sensitive HPLC method for measurement of vitamin A and β-carotene in sera of healthy individuals. This study was conducted at chemistry and biochemistry department, college of medicine, Al-Nahrain University, Baghdad, Iraq from July2014 to March 2015; a cross-linked polymer was synthesized by condensation reaction between mixture of glycerol and triethanol amine with maleic anhydride. The resulted polymer have high rigidity and easily grinded, with high stability and used as stationary phase for HPLC column. Vitamin A and β-carotene were measured using by improved gradient reversed phase HPLC technique. Reproducible determination with highly sensitive detection was attained by HPLC with UV-Visible detection. The detection limit of vitamin A and β-carotene were 0.05and 0.1µg/ml respectively. Fifty healthy volunteers aged 25.45±6.54 years were included in this study. All participant and exclude taking supplement of multi vitamins for the last two months. The method gives a good linearity ranges between 0.05 to 15µg/ml for Vitamin A and β-Carotene. The CVs% of within-day precision for vitamin A was 1.41-2.42% and β-carotene was 0.81-1.17%. While the analytical recoveries were98.2% for vitamin A and 96.8% for β-carotene.
NOOR MUSTAFA ALI
triethanolamine-glycerol-maleate, vitamin A, β-carotene, HPLC.
350-358