International Journal of Pharma and Bio Sciences
ijpbs.net
editorijpbs@rediffmail.com (or) editorofijpbs@yahoo.com (or) prasmol@rediffmail.com
10.22376/ijpbs.2019.10.1.p1-12
Volume 8 Issue 1
2017 (January - March)
Regeneration of plantlets from leaf derived Callus of ammi majus-a medicinal plant
An efficient protocol was developed for high frequency plant regeneration from leaf explants of lessThan i greaterThan Ammi majus lessThan /i greaterThan . lessThan i greaterThan Ammi majus lessThan /i greaterThan L. (Apiaceae) is a herb of pharmaceutical as well as ornamental interest and used in the treatment of leucoderma. Since in lessThan i greaterThan Ammi majus lessThan /i greaterThan the seed set and germination is poor attempts were made to propagate the plants through tissue culture. The callus from cotyledonary leaves obtained on Murashige and Skoog's (MS) medium supplemented with a-indoleacetic acid (IAA) + Kinetin (Kn) + Casein hydrolysate (CH), differentiated shoots in 100% cultures when adenine and glutamine were added to the above medium. Plantlets resulted on MS medium with indolebutyric acid (IBA) and glutamine.
SANJEEV KUMAR
Ammi majus, callus, regeneration, plantlet
416-419