International Journal of Pharma and Bio Sciences
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10.22376/ijpbs.2019.10.1.p1-12
Volume 8 Issue 4
2017 (October - December)
Phytochemical screening and in vitro antioxidant potential of hydroalcoholic extract from the aerial parts of bryonopsis laciniosa
The aim of present study was to estimate the total phenolic and flavonoid content and also to evaluate in-vitro antioxidant potential of hydroalcoholic extract of aerial parts of lessThan i greaterThan Bryonopsis laciniosa (HAEBL). lessThan /i greaterThan Antioxidant activity was assessed by using 2, 2- diphenyl-1-picryl-hydrazyl (DPPH) assay, ferric reducing power, Hydrogenperoxide radical scavenging assay, nitric oxide scavenging assay, superoxide radical scavenging assay and hydroxyl radical scavenging assay using ascorbic acid as standard antioxidant. The data obtained from all these models clearly suggested that the antioxidant activity of lessThan i greaterThan HAEBL lessThan /i greaterThan was significant and dose dependent. The extract was found to scavenge effectively the free radicals such as peroxides, superoxides and hydroxyl radicals. The total phenolic and flavonoid contents were determined by established methods and were found to be 126.1 mg GAE/g and 262.6 mg of CE/g in gallic acid and catechin equivalents respectively. The antioxidant activities may be attributed to the presence of significant amounts of phenolic and flavonoid compounds.
LENIN BABU VALLURI , Dr. K. S. MURALI KRISHNA AND CH. MADHU
Bryonopsis laciniosa, Hydroalcoholic extract, Phytochemical Screening, TLC, Total phenolic content, Total flavonoid content, Antioxidant activity.
109-118