The herbal medicinal plant Pittosporum floribundum is a rare mountain tree species of Seshachalam Hill Ranges of Eastern Ghats. Local herbalists use this species in healing skin diseases, arthritis, inflammation, bronchitis, leprosy, narcotic and antidote to snake venom. Phytochemical screening reveals the presence of tannins, alkaloids, saponins, glycosides, fixed oils, steroids, flavonoids and phenols mainly in water, alcohol and methanol extracts of leaf, bark, seed and fruit. Methanol extracts of bark showed more effective inhibition zones against all selected bacterial strains and two fungal strains at 20-30mg/ml with 16-25mm Zone of Inhibition than leaf, seed and fruit. Minimum Inhibitory Concentration ranges between 0.312- 1.25 mg against all microbial strains.