The antimicrobial property in the extracts of Blechnum orientale L. has been evaluated on clinically isolated bacterial pathogens such as Staphylococcus aureus, Bacillus subtilis, Salmonella typhi, Mycobacterium luteus, Pseudomonas aeruginosa and Escherichia coli. Also the antifungal activity was tested on Candida albicans and Aspergillus flavus. The pet-ether, chloroform, methanol and aqueous extracts were used for the study of antimicrobial activity by agar well diffusion, micro titre method and tube dilution methods. Results indicated that all the four extracts were effective against E. coli, P. aeruginosa and C. Albicans, . Aqueous extract showed relatively higher zone of inhibition against E. coli, P. aeruginosa and C. albicans while S. aureus, B. subtilis, S. typhi, M. luteus and A. flavus were not found to be sensitive to all the extracts.