International Journal of Pharma and Bio Sciences
    ISSN 0975-6299

Int J Pharm Bio Sci Volume 11 Issue 4, 2020 (October-December), Pages:5-13

In vivo in Vitro Antioxident Study of Ficus Bengalensis Linn

Fegade Sachin A* and Siddaiah M

Herbal drugs and herbal formulations are frequently considered to be less toxic and free from side effects than the synthetic one. Indian and other ancient civilizations were leveraging these plants for curing and treating various diseases and ailments. The imposing banyan tree of poetry and legend is a store-house of invaluable remedies for some of the deadliest diseases. Ficus bengalensis Linn (Moraceae) is commonly known as Banyan tree or Bargad. It possesses several medicinal properties in ayurveda and other traditional systems of medicines It is renowned for the presence of polyphenols and flavonoids in plants, generally responsible for their vibrant antioxidant activity. The present study focused on the antioxidant activity of ethanol, aqueous extract and chloroform fraction Invivo and in vitro respectively of aerial parts of Ficus bengalensis Linn found to be. The total phenolic contents of alcoholic and aqueous extracts of aerial parts of Ficus bengalensis Linn was found to 56.8 mg and 113.1 mg of gallic acid equivalent (GAE)/g respectively and total flavonoids content of both test extracts of Ficus bengalensis Linn was found to be and 8.3 mg equivalent of quercetin /gm of the dry weight basis. The obtained amount of total phenolic and flavonoids in the tested extracts indicated that the extracts possess high antioxidant potential and In vitro methods have proven the effectiveness of the tested extracts and fractions of Ficus bengalensis Linn compared to the reference standard antioxidant ascorbic acid. Therefore, Ethanol, aqueous extracts and chloroform fraction of Ficus bengalensis Linn aerial parts indicates its ability to scavenge free radicals, thereby preventing inhibiting peroxidation chain reaction and thus provide protection against the development of diabetes which may be due to the presence of phenolic and flavonoid. Consequently it may be assumed that the antioxidant activity of the extract might play an important role for anti-hyperglycemic activity of the plant extract.

Keywords: Antioxidant, Ficus benghalensis Linn, DPPH, Free radical, phenolic content, flavonoids
Full HTML:
  1. Fleischer T, Sarkodie J, Komlaga G, Kuffour G, Dickson R, Mensah M. Hypoglycaemic and Antioxidant Activities of the Stem Bark of Morinda Lucida Benth in Streptozotocin – Induced Diabetic Rats. Phcog Commn. 2011;1(2):23-9. doi: 10.5530/pc.2011.2.5.
  2. Yadav YC  Srivastava DN , Saini V. Seth AK , In-Vitro Antioxidant Activity of Methanolic Extraction of Ficus Benghalensis L. Latex  2011.Pharmacologyonline 1: 140-148
  3. Satish A.  Punith Kumar R.  Rakshith D. Satish S. Faiyaz Ahmed  Antimutagenic and antioxidant activity of  Ficus benghalensis stem bark and Moringa oleifera root extract. International Journal of Chemical and Analytical Science 2013;4(2):45-48
  4. Ali B, Mujeeb M,Aeri V,Miri SR,  M. Faiyazuddin F. Shakeel Anti-inflammatory and antioxidant activity of Ficus carica Linn. Leaves Natural Product Research, 2012;5(26):460-65.
  5. Ghosh S, Shirwaikar A, Rao PG. Effect of Ficus bengalensis L. leaves on wound healing in rats. Indian J Nat Prod. 2004;3(5):45-8.
  6. Fridovich I. Superoxide radical and superoxide dismutases. Annu Rev Biochem. 1995;64(1):97-112. doi: 10.1146/
  7. Ghosh MN. Fundamentals of experimental pharmacology. Kolkata: Hilton Publishing and company. p. 190-195http://www; 2005. 3rd ed. Available from:;year=2007;volume=39;issue=4;spage=216;epage=216;aulast=Ghosh.
  8. Giugliano D, Ceriello A, Paolisso G. Diabetes mellitus, hypertension, and cardiovascular disease: which role for oxidative stress? Metabolism. 1995;44(3):363-8. doi: 10.1016/0026-0495(95)90167-1, PMID 7885282.
  9. Göke B, Herrmann-Rinke C. The evolving role of alpha-glucosidase inhibitors. Diabetes Metab Rev. 1998;14;Suppl 1:S31-8. doi: 10.1002/(sici)1099-0895(199809)14:1+<s31::aid-dmr226>;2-v. PMID 9816485.
  10. Gupta R, Sharma AK, Sharma MC, Dobhal MP, Gupta RS. Evaluation of antidiabetic and antioxidant potential of lupeol in experimental hyperglycaemia. Nat Prod Res. 2012;26(12):1125-9. doi: 10.1080/14786419.2011.560845, PMID 22043924.
  11. Jain SJ, Khan T. Phytoconstituents from aerial roots of Ficus benghalensis Linn. Indo Am J Pharm Res. 2015;5(10):3261-3280
  12. Jakus V. The role of free radicals, oxidative stress and antioxidant systems in diabetic vascular disease. Bratisl Lek Listy. 2000;101(10):541-51. PMID 11218944.
  13. Karuppasamy R, Veerabahu RM. In-vitro antioxidant activity of Dioscorea tomentosa Koen ex Spreng. Asian Pac J Trop Biomed. 2012;1-5.
  14. Kataja-Tuomola MK, Kontto JP, Männisto S, Albanes D, Virtamo J. Intake of antioxidants and risk of Type 2 diabetes in a cohort of male smokers. Eur J Clin Nutr. 2011;65(5):590-7. doi: 10.1038/ejcn.2010.283, PMID 21245884.
  15. Kaur C, Binoy G, Deepa N, Balraj S, Kapoor HC. Antioxidant status of fresh and processed tomato. J Food Sci Technol. 2004;41:479-80.
  16. Kaur K, Bedi G, Kaur M, Vij A, Kaur I. Lipid peroxidation and the levels of antioxidant enzymes in coronary artery disease. Indian J Clin Biochem. 2008;23(1):33-7. doi: 10.1007/s12291-008-0008-4, PMID 23105716.
  17. Lewis P, Stefanovic N, Pete J, Calkin AC, Giunti S, Thallas-Bonke V, Jandeleit-Dahm KA, Allen TJ, Kola I, Cooper ME, de Haan JB. Lack of the antioxidant enzyme glutathione Peroxidase-1 accelerates atherosclerosis in diabetic apolipoprotein E–deficient mice. Circulation. 2007;115(16):2178-87. doi: 10.1161/CIRCULATIONAHA.106.664250, PMID 17420349.
  18. Ambi AA, Idrees AI. Pharmacognostic studies of the leaf of Ficus benghalensis Linn. Trends Sci Technol J. April 2017;2(1):165-8.
  19. Etratkhan Z, Seifalizadesh Y, Sayed E. Antioxidant activity and phytochemical screening of Ficus benghalensis aerial roots fractions. J Rep Pharm Sci. 2019;6(1):24-7.
  20. Kumaran A, Joel Karunakaran R. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT - Food Science and Technology. 2007;40(2):344-52. doi: 10.1016/j.lwt.2005.09.011.
  21. NazeerAhamed KFH, Mukherjee K, Bandyopadhyay A. Mukherjeea PK. Antioxidant effect of Cytisus scoparius against carbon tetrachloride treated liver injury in rats. J Ethnopharmacol. 2007;109(1 (3)):41-7.
  22. Gospodaryov D, Lushchak V. Oxidative stress: cause and consequence of diseases chapter ·; April 2012. doi: 10.5772/38093.
  23. Beauchamp C, Fridovich I. Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem. 1971;44(1):276-87. doi: 10.1016/0003-2697(71)90370-8, PMID 4943714.
  24. Chelikani P a, I. Fita b and P. C. Loewena, Diversity of structures and properties among catalases. CMLS Cell Mol Life Sci. 1420–682X/04/020192-17 DOI 10.1007/s00018-003-3206-5;61 (2004):192-208.Dengiz GO, Odabasoglu F, Halici Z, Cadirci E, Suleyman H. Gastroprotective and antioxidant effects of montelukast on indomethacin-induced gastric ulcer in Rats 2007. J Pharmacol Sci. 2007 September;105(1):94-102. doi: 10.1254/jphs.fp0070122, PMID 17895592.
  25. Hansawasdi C, Kawbata J. Kasai amylase inhibitors from Roselle (Hibiscus sabdariffa linn.) tea bioscience, biotechnology, and biochemistry. Available from: Vol. 64(5); 2000–.
[Download PDF]
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy
Pharmaceutical Fields
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Pharmaceutics
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Novel drug delivery system
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Nanotechnology
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Pharmacology
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Pharmacognosy
© Copyright 2009-2015 IJPBS, India. All rights reserved. Specialized online journals by ubijournal. Website by Ubitech Solutions
         Home I Contact I Terms & Conditions