International Journal of Pharma and Bio Sciences
    ISSN 0975-6299

Int J Pharm Bio Sci Volume 12 Issue 1, 2021 (January-March), Pages:66-72

Assessment of Hepatoprotective Activity of Justicia adhatoda L. Herbal Extracts Using Carbon Tetrachloride Influenced Hepatotoxicity in Rats Model.

Gaurav Kumar Sharma*and Sarita Sharma

The home grown medications, utilized in Indian frameworks of meds, are, in any case, being professed to be compelling and good for liver infection. The current examination has endeavored to exhibit the hepatoprotective movement of different natural concentrates of Justicia adhatoda L. by CCl4 incited hepatotoxicity in the Albino rats (Wister strain). The parameter like enzyme study (SGOT, SGPT, SALP, Serum bilirubin (total and direct) with histopathological studies have been carried out and the extent of regenerative changes have been observed. It was observed that the Chloroform concentrate of Justicia adhatoda L. at 200mg/kg of body weight indicated a critical decrease in raised degree of SGOT, SGPT, SALP and bilirubin. While the Petroleum ether and Aqueous concentrate showed moderate decrease in catalyst level. Ethanol concentrates did not show any huge decrease in protein level, demonstrating no movement. The hepatoprotective movement was upheld by minuscule histopathological assessment of liver tissue. Since after effects of biochemical investigations of blood tests of carbon tetrachloride rewarded rodents indicated critical increment in the degrees of serum compound exercises, mirroring the liver injury brought about by CCl4 and blood tests from the creatures rewarded with the Chloroform concentrate of Justicia adhatoda L. have demonstrated critical abatement in the degrees of serum markers, showing rewarded liver have been found to have noticeable hepatocytes with increasingly regenerative movement, which implies better hepatoprotective action when contrasted with different gatherings. Though Petroleum ether and Aqueous concentrate of Justicia adhatoda L. rewarded liver found to have the moderate level of greasy changes, which demonstrated moderate hepatoprotective action when contrasted with different gatherings. Considering the eventual outcomes of various biochemical boundaries, all the arrangements have demonstrated important hepatoprotective development for all intents and purposes indistinguishable with LIV-52.The best hepatoprotective activity was found with Chloroform concentrate of Justicia adhatoda L.

Keywords: Hepatoprotective activity, Carbon tetrachloride (CCl4), LIV-52, Justicia adhatoda L.
Full HTML:


1.       Subrata D, Ravishankar B, Bhavsar GC. Plants with hepatoprotective activity - a review. Indian Drugs. 1993;30(9):355.

2.       Kokate CK, Purohit AP, Gokhale. Text book of pharmacognosy. 4th ed. Pune: NiraliPrakashan; 1996.

3.       Ram VJ. Herbal Preparations as a source of hepatoprotective agents. Drug News Perspect. 2001;14(6):353-63. PMID 12813598.

4.       Nadeem M, Dandiya PC, Pasha KV, Imran M, Balani DK, Vohara SB. Hepatoprotective activity of some herbal formulations available in India. Indian Drugs. 1996;33(9):390.

5.       Kuruvilla A. Herbal formulations AS Pharma- cotherapeutic agents. J Ethnopharmacol. 1999, Sep;66(3):355-61.

6.       Zimmerman HJ. Effects of various agents in Hepatotoxicity. Craft. 1978;33:178.

7.       Kissane JM. Andersson’s pathology. 9th ed. Vol. 1. Toronto: C.V. Mosby Company; 1990. p. 216.

8.       Atal CK. Chemistry and Pharmacology of vasicine: A new oxytocin and abortifacient. Indian Drugs. 1980;15:15-8.

9.       Kirtikar KR, Basu BD. Indian Medicinal plants. 2nd ed. Delhi. Bishensinghmahendra pal singh publication; 1975. p. 37-58.

10.     Adnan M, Hussain J, Shah MT, Ullah F, Shinwari JK, Bahadar A, Khan AL. Proximate and Nutrient Composition of Medicinal Plants of Humid and Sub-humid regions in Northwest Pakistan. J Med Plants Res. 2010;4:339-45. doi: 10.5897/JMPR09.505.

11.     Atta-Ur-Rahman SHM, Ahmad VU. Pakistan encyclopaediaplantamedica. Vol. 1. Karachi: Hamdard Foundation Press; 1986. p. 181-7.

12.     Roberts E. Vegetable materia medica of India and Ceylon. Colombo: Plate Limited; 1931. p. 269-78.

13.     Lal SD, Yadav BK. Folk medicines of kurukshetra district (Haryana), India. Econ Bot. 1983;37(3):299-305. doi: 10.1007/BF02858885.

14.     Shah NC, Joshi MC. An Ethnobotanical study of the Kumaon region of India. Econ Bot. 1971;25(4):414-22. doi: 10.1007/BF02985209.

15.     Pushpangadan P, Nyman U, George V. Glimpses of Indian ethnopharmacology. KER: Tropical Botanic Garden and Research Institute; 1995. p. 309-83.

16.     Bhat VS, Nasavatl DD, Mardikar BR. Adhatodavasica-an Ayurvedic plant. Indian Drugs. 1978;15:62-6.

17.     Dymock W, Warden CJH, Hooper D. PharmacographiaIndica, A history of the principal drugs of vegetable origin. Paul, Trench: Trübner and Co, Ltd; 1890. London, pp. 188-212.

18.     Hukkeri VI, Shastri R, Karadi RV, Savadi RV. Hepatoprotective activity of fruit pulp of Annonareticilata carbon tetrachloride-induced toxicity. Indian Drugs. 2004;41(11):684-9.

19.     Galighor AE, Kozloff EN. Essentials of practical Micro Technique. 2nd ed. New York: Lea & Febiger; 1976.

20.     Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am J Clin Pathol. 1957;28(1):56-63. doi: 10.1093/ajcp/28.1.56, PMID 13458125.

21.     Varley H, Gowenlock AH, Bell M. Ed. Practical clinical biochemistry. 5th ed. London: William Heinemann Medical Books Ltd; 1994. p. 601-5, 891-907. Record Number : 19552200856.

22.     Anyasor GN, Moses N, Kale O. Hepatoprotective and hematological effects of JusticiasecundaVahl leaves on carbon tetrachloride induced toxicity in rats. Biotech Histochem. 2020 Jul;95(5):349-59. doi: 10.1080/10520295.2019.1700430. PMID 31969022.

23.     Pareek A, Godavarthi A, Issarani R, Nagori BP. Antioxidant and hepatoprotective activity of Fagonia schweinfurthii (Hadidi) Hadidi extract in carbon tetrachloride induced hepatotoxicity in HepG2 cell line and rats. J Ethnopharmacol. 2013 Dec 12;150(3):973-81. doi: 10.1016/j.jep.2013.09.048. PMID 24140589.

24.     Roy CK, Das AK. Comparative evaluation of different extracts of leaves of Psidium guajava Linn. forhepatoprotective activity. Pak J Pharm Sci. 2010 Jan;23(1):15-20. PMID 20067861.

25.     Kalegari M, Gemin CA, Araújo-Silva G, Brito NJ, López JA, TozettoSde O, Almeida Md, Miguel MD, Stien D, Miguel OG. Chemical composition, antioxidant activity and hepatoprotective potential of Roureainduta Planch. (Connaraceae) against CCl4-induced liver injury in female rats. Nutrition. 2014 Jun;30(6):713-8. doi: 10.1016/j.nut.2013.11.004. PMID 24800671.

26.     Qureshi NN, Kuchekar BS, Logade NA, Haleem MA. Antioxidant and hepatoprotective activity of Cordia macleodii leaves. Saudi Pharm J. 2009 Oct;17(4):299-302. doi: 10.1016/j.jsps.2009.10.007. PMID 23960714, PMCID PMC3731011.

27.     Fernando CD, Soysa P. Total phenolic, flavonoid contents, in-vitro antioxidant activities and hepatoprotective effect of aqueous leaf extract of Atalantiaceylanica. BMC Complement Altern Med. 2014 Oct 14;14:395. doi: 10.1186/1472-6882-14-395, PMID 25311044, PMCID PMC4210483.

28.     Onochie AU, Oli AH, Oli AN, Ezeigwe OC, Nwaka AC, Okani CO, Okam PC, Ihekwereme CP, Okoyeh JN. The Pharmacobiochemical Effects of ethanol Extract of Justicia secunda Vahl Leaves in RattusNorvegicus. J Exp Pharmacol. 2020 Nov 2;12:423-37. doi: 10.2147/JEP.S267443, PMID 33173354, PMCID PMC7646487.

29.     Mohamed MA, Eldin IM, Mohammed AE, Hassan HM. Effects of Lawsonia inermis L. (Henna) leaves’ methanolic extract on carbon tetrachloride-induced hepatotoxicity in rats. J Intercult Ethnopharmacol. 2016;5(1):22-6. doi: 10.5455/jice.20151123043218, PMID 27069719, PMCID PMC4805143.

30.     Molehin OR, Oloyede OI, Idowu KA, Adeyanju AA, Olowoyeye AO, Tubi OI, Komolafe OE, Gold AS. White butterfly (Clerodendrumvolubile) leaf extract protects against carbon tetrachloride-induced hepatotoxicity in rats. Biomed Pharmacother. 2017 Dec;96:924-9. doi: 10.1016/j.biopha.2017.12.005. PMID 29224792.

31.     Brai BI, Adisa RA, Odetola AA. Hepatoprotective properties of aqueous leaf extract of Persea americana, Mill (Lauraceae) ’avocado’ against CCL4-induced damage in rats. Afr J Tradit Complement Altern Med. 2014 Jan 28;11(2):237-44. doi: 10.4314/ajtcam.v11i2.2, PMID 25435602, PMCID PMC4202626.

32.     Jain A, Soni M, Deb L, Jain A, Rout SP, Gupta VB, Krishna KL. Antioxidant and hepatoprotective activity of ethanolic and aqueous extracts of MomordicadioicaRoxb. leaves. J Ethnopharmacol. 2008 Jan 4;115(1):61-6. doi: 10.1016/j.jep.2007.09.009. PMID 17983713.

33.     Tong J, Yao X, Zeng H, Zhou G, Chen Y, Ma B, Wang Y. Hepatoprotective activity of flavonoids from Cichorium glandulosum seeds in vitro and in vivo carbon tetrachloride-induced hepatotoxicity. J Ethnopharmacol. 2015 Nov 4;174:355-63. doi: 10.1016/j.jep.2015.08.045. PMID 26320690.

34.     Li L, Zhou YF, Li YL, Wang LL, Arai H, Xu Y. In vitro and in vivo antioxidative and hepatoprotective activity of aqueous extract of Cortex Dictamni. World J Gastroenterol. 2017 Apr 28;23(16):2912-27. doi: 10.3748/wjg.v23.i16.2912, PMID 28522909, PMCID PMC5413786.

[Download PDF]
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy
Pharmaceutical Fields
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Pharmaceutics
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Novel drug delivery system
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Nanotechnology
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Pharmacology
Welcome to IJPBS,Pharmaceutics, Novel, drug, delivery, system, Nanotechnology, Pharmacology, Pharmacognosy Pharmacognosy
© Copyright 2009-2015 IJPBS, India. All rights reserved. Specialized online journals by ubijournal. Website by Ubitech Solutions
         Home I Contact I Terms & Conditions