International Journal of Pharma and Bio Sciences
    ISSN 0975-6299

Int J Pharm Bio Sci Volume 12 Issue 3, 2021 (July-September), Pages:48-57

GC-MS Analysis of Bioactive Components in the Methanolic Extract of Graviola (Gishta) Seeds and Their Pharmacological Activities

Harikrishna Ramaprasad Saripalli and Prasanna Kumar Dixit

Bioactive substances are the leading-edge molecules in both the pharmaceutical and nutraceutical industries. Recently, bioactive compounds are gaining much importance for their enhanced resistance to numerous diseases and in boosting people’s health by both traditional and modern ways of administration. In traditional medicine, many plants have provided valuable clues for being used as potential antiparasitic, antimalarial, leishmanicidal, anti-tumor, fungicidal, and antibacterial compounds. Still, several plants are yet to be screened for their abilities to stand forth as medicinal plants. Locally, Graviola (Gishta) fruits are being used for dietary purposes and traditional medicinal purposes, but their beneficial effects have never been attributed to the presence of active bio compounds.  Hence, the present investigation was undertaken to study Gas Chromatography-Mass Spectrometry (GC-MS) analysis of methanolic extracts of Graviola seeds.  GC-MS analytical method was employed with specific conditions such as Ion source temp 200°C, Interface temp 240°C, Scan range 40 – 1000 m/z,   MS start time 5(min), MS end time 35 (min), Ionization EI (-70ev), Scan speed 2000. The methanolic extracts of Graviola seeds exhibited the presence of seventy types of bioactive compounds.  The foremost important compounds identified are 1H-Cyclopenta [l, 3] cyclopropa [l, 2] benzene, octa hydro-7-methyl-3-methylene-4-( 1-methyl ethyl) -, [3aS-( 3a. alpha.,3b. beta., 4. beta., 7. alpha.,7 aS*)]-Bicyclo [4. 4. 0] dec-l-ene, 2-isopropyl-5-methyl-9-methylene-. gamma.-Muurolene. tetracosane, phytol and squalene. In an exceeding mass spectral view, each of the compounds has been identified based on their retention time and % of peak area(s). Analytes with high peak areas showed various pharmacological properties such as antibacterial, antifungal, anticancer, etc.,) which provided experimental evidence for its traditional medicinal claim. The spectra of phytochemical constituents found in the seed extracts signify the potential of the Graviola plant as a source of therapeutic agent(s) and may provide leads in the ongoing search for a novel phytotherapeutic agent.  


Keywords: : Bioactive compounds, Methanolic extract, Gas Chromatography-Mass Spectrometry, Pharmacological activity, and Graviola seeds.
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  1. Kesava RB, Usha RG. GC-MS analysis of volatile components in petroleum ether extracts of Coldenia procumbens Linn. Int J Pharm Biol Sci. 2016;7(2):241-5.
  2. Edwards S, Tadesse M, Demissew S, Hedberg I (eds.). 2000. Flora of Ethiopia and Eritrea, Volume 2, part 1. The national herbarium Addis Ababa, Ethiopia, and Uppsala, Sweden. Pp. 10-2.
  3. Graviola (Soursop) [Internet]. [updated 2016 Nov 15; cited 2019 January 30]. Available form:
  4. Saripalli H, Dixit P. Studies on morphological features and biological activities of the genus Annona of Ethiopia, N.E. Africa with a special emphasis on Graviola: a review. Int J Sci Eng Res (IJSR). 2016;5(2):821-7. doi: 10.21275/v5i2.3021602.
  5. Ruppercht JK, Chang CJ, Cassady JM, McLaughlin JL. Heterocycles. 1982; 24: 1197-201.
  6. Waltor, H.F. Principle and methods of Chemical Analysis, Prentice Hall of India Pvt. Ltd., New Delhi, 1971.
  7. A.N.M. Alamgir. Therapeutic Use of Medicinal Plants and their Extracts: Volume 2: Phytochemistry and Bioactive Compounds Volume 74 of Progress in Drug Research, Springer, 2018. p. 173-176. [cited 2021 March 15]  Available from:
  8. Cruz ASP. Anthelmintic effect of Solanum lycocarpum in mice infected with Aspiculuris tetraptera. The journal of American science 2008; 4(3): 75-79.
  9. Wang GS, Han J, Zhao LW, Jiang DX, Liu YT, Liu XL. Anthelmintic activity of steroidal saponins from Paris polyphylla. Phytomedicine 2010; 17: 1102-1105.
  10. Shaibani TRMA, Phulan MS, Shiekh M. Anthelmintic activity of Fumaria parviflora  (Fumariaceae) against gastrointestinal nematodes of sheep. Int. J. Agric. Biol. 2009; 11: 431–436.
  11. Cowan MM. Plant products as antimicrobial agents. Clinical microbiology reviews 1999; 12(4): 564-582.
  12. Kumar R, Sharma RJ, Bairwa K, Roy RK, Kumar A. Pharmacological review on natural antidiarrhoel agents. Der Pharma Chemica 2010; 2(2): 66-93.
  13. Compound Methyl linoleate Product ID: FDB012761 [Internet]. Version. 1.0. [created 2010 April 08; updated 2020 November 17; cited 2021 March 12]. Available from:
  14. Sutar N, Garai R, Sharma US, Sharma UK. Anthelmintic activity of Platycladus orientalis leaves extract. International Journal of Parasitology Research 2010; 2(2): 1-3.
  15. Eloff JN. Which extractant should be used for the screening and isolation of antimicrobial components from plants. Journal of Ethnopharmacology 1998; 60: 1–8.
  16. Audu SA, Mohammed I, Kaita HA. Phytochemical screening of the leaves of Lophira lanceolata (Ochanaceae). Life Science Journal 2007; 4(4): 75-79.
  17. Prashant Tiwari, Bimlesh Kumar, Mandeep Kaur, Gurpreet Kaur and Harleen Kaur (2011). Phytochemical screening and Extraction: A Review. Internationale Pharmaceutica Sciencia. Vol 1 Issue 1:, pp 98- 106,
  18. Javed H, Azimullah S, Haque ME, Ojha SK. Cannabinoid Type 2 (CB2) receptors activation protects against oxidative stress and neuroinflammation associated dopaminergic neurodegeneration in rotenone model of Parkinson’s disease. Front Neurosci. 2016;10:321. doi: 10.3389/fnins.2016.00321, PMID 27531971.
  19. Cho JY, Chang HJ, Lee SK, Kim HJ, Hwang JK, Chun HS. Amelioration of dextran sulphate sodium-induced colitis in mice by oral administration of beta-caryophyllene, a sesquiterpene. Life Sci. 2007;80(10):932-9. doi: 10.1016/j.lfs.2006.11.038, PMID 17188718.
  20. Pieri FA, Souza MC, Vermelho LL, Vermelho ML, Perciano PG, Vargas FS, Borges AP, da Veiga-Junior VF, Moreira MA. Use of β-caryophyllene to combat bacterial dental plaque formation in dogs. BMC Vet Res. 2016;12(1):216. doi: 10.1186/s12917-016-0842-1, PMID 27716286.
  21. Swamy MK, Akhtar MS, Sinniah UR. Antimicrobial properties of plant essential oils against human pathogens and their mode of action: an updated review. Evid Based Complement Alternat Med. 2016;   3012462. doi: 10.1155/2016/3012462, PMID 28090211.
  22. Yamaguchi M, Levy R. β-caryophyllene promotes osteoblastic mineralization and suppresses osteo clasto genesis and adipogenesis in mouse bone marrow cultures in vitro, 2016. from
  23. Dias DS, Fontes LB, Crotti AE, Aarestrup BJ, Aarestrup FM, da Silva Filho AA, Corrêa JO. Copaiba oil suppresses inflammatory cytokines in splenocytes of C57BL/6 mice induced with experimental autoimmune encephalomyelitis (EAE). Molecules. 2014; 19(8): 12814-26. doi: 10.3390/molecules190812814, PMID 25153880.
  24. Russo EB. Taming THC: potential cannabis synergy and phyto-cannabinoid - terpenoid entourage effects. Br J Pharmacol. 2011; 163: 1351.
  25. Katsuyama S, Mizoguchi H, Kuwahata H, Komatsu T, Nagaoka K, Nakamura H, Bagetta G, Sakurada T, Sakurada S. Involvement of peripheral cannabinoid and opioid receptors in β-caryophyllene-induced antinociception. Eur J Pain. 2013;17(5):664-75. doi: 10.1002/j.1532-2149.2012.00242.x, PMID 23138934.
  26. Ncube NS, Afolayan AJ, Okoh AI. Assessment techniques of antimicrobial properties of natural compounds of plant origin: current methods and future trends. African Journal of Biotechnology 2008; 7 (12): 1797-1806.
  27. Remington JP. Remington: The science and practice of pharmacy, 21st edition, Lippincott Williams & Wilkins, 773-774.
  28. Handa SS, Khanuja SPS, Longo G, Rakesh DD. Extraction Technologies for Medicinal and Aromatic Plants. International centre for science and high technology, Trieste, 2008, 21-25.
  29. Das K, Tiwari RKS, Shrivastava DK. Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. Journal of Medicinal Plants Research 2010; 4(2): 104 - 111.
  30. Obasi NL, Egbuonu ACC, Ukoha PO, Ejikeme PM. Comparative phytochemical and antimicrobial screening of some solvent extracts of Samanea saman pods. African journal of pure and applied chemistry 2010; 4(9): 206-212.
  31. Sermakkani M. and Thangapandian. V.  GC-MS Analysis of Cassia Italica leaf methanol extract. Asian Journal of Pharmaceutical and Clinical Research Vol 5, Issue 2, 2012; 90-94.
  32. Gomathi, D., Kalaiselvi, M., Ravikumar, G. et al. GC-MS analysis of bioactive compounds from the whole plant ethanolic extract of Evolvulus alsinoides (L.) L. J Food Sci Technol 521212–1217 (2015).
  33. Karthika Krishnamoorthy and Paulsamy Subramaniam. Phytochemical Profiling of Leaf, Stem, and Tuber Parts of Solena amplexicaulis (Lam.) Gandhi Using GC-MS. International Scholarly Research Notices, Volume 2014, Article ID 567409, 1-14.




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