International Journal of Pharma and Bio Sciences
 
 
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ORIGINAL RESEARCH ARTICLE
Int J Pharm Bio Sci Volume 1 Issue 3, 2010 (July - September), Pages:58-69

Toxicological Studies of Purified Soymida Febrifuge and Acacia Nilotica Exudate Gum

Pravin R. Bhushette, Satish V. Rojekar and Uday S. Annapure
DOI: http://dx.doi.org/10.22376/ijpbs.2021.12.3.P58-69
Abstract:

It is crucial to determine the safety profile of components used in food or pharma products. Acacia Nilotica exudate gum (ANG) and Soymida febrifuga exudate gum (SFG) have shown similar acacia gum properties, but their safety profile is not studied.  In the present study, acute oral toxicity of ANG and SFG was investigated in female Sprague-Dawley rats for 14 days. The study aims to investigate the ANG and SFG exudate gums safety in terms of adverse reactions and mortality. Bodyweight and hematological parameters were monitored from zero-day in the interval of 2 and 7 days, respectively. This showed no significant reduction in ANG and SFG fed groups' body weight compared to the control group. Also, hematological parameters in the animal fed with ANG and SFG showed no considerable change compared to the control group. The significant increase in ALT and AST in the liver homogenate following the administration of 2000 mg/kg aqueous ANG because of the enzyme induction by the high dose of the exudate gums synthesizes the increased enzymes. The histopathological study showed no significant changes in the liver and other organ sections of animals fed with ANG and SFG compared to the control group. The LD50 of SFG and ANG were found to be more than 2000 mg/kg, and it suggested that these gum exudates were safe for food and pharmaceutical applications.

Keywords: Exudate Gum Toxicity, Haematological study; Histopathological study; Acacia Nilotica; Soymida febrifuga
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REFERENCES

 

  1. Verbeken D, Dierckx S, Dewettinck K. Exudate gums: occurrence, production, and applications. Appl Microbiol Biotechnol. 2003;63(1):10-21. doi:10.1007/s00253-003-1354-z

2.     Krishnan S, Bhosale R, Singhal RS. Microencapsulation of cardamom oleoresin: Evaluation of blends of gum arabic, maltodextrin and a modified starch as wall materials. Carbohydr Polym. 2005;61(1):95-102. doi:10.1016/j.carbpol.2005.02.020
3.     Chattopadhyaya S, Singhal RS, Kulkarni PR. Oxidised starch as gum arabic substitute for encapsulation of flavours. Carbohydr Polym. 1998;37(2):143-144. doi:10.1016/S0144-8617(98)00054-X
4.     Bhushette PR, Annapure US. Physicochemical, functional and rheological investigation of Soymida febrifuga exudate gum. Int J Biol Macromol. 2018;111(C):1116-1123. doi:10.1016/j.ijbiomac.2018.01.117
5.     Bhushette PR, Annapure US. Comparative study of Acacia nilotica exudate gum and acacia gum. Int J Biol Macromol. 2017;102:266-271. doi:10.1016/j.ijbiomac.2017.03.178
6.     Erum A, Bashir S, Saghir S, et al. Acute toxicity studies of a novel excipient arabinoxylan isolated from Ispaghula (Plantago ovata) husk. Drug Chem Toxicol. 2015;38(3):300-305.
7.     Amien AI, Fahmy SR, Abd-Elgleel FM, Elaskalany SM. Renoprotective effect of Mangifera indica polysaccharides and silymarin against cyclophosphamide toxicity in rats. J Basic Appl Zool. 2015;72:154-162.
8.     Divvela HND, Duppala L, Kolapalli VRM. Isolation and acute oral toxicity studies of Araucaria heterophylla novel natural polysaccharide gum in albino mice. World J Pharm Pharm Sci. 2016;5(10):702-711.
9.     Velusami CC, Boddapati SR, Hongasandra Srinivasa S, et al. Safety evaluation of turmeric polysaccharide extract: assessment of mutagenicity and acute oral toxicity. Biomed Res Int. 2013;2013.
10.     Kean T, Thanou M. Biodegradation, biodistribution and toxicity of chitosan. Adv Drug Deliv Rev. 2010;62(1):3-11.
11.     Jia Y, Hu Y, Zhu Y, et al. Oligoamines conjugated chitosan derivatives: Synthesis, characterization, in vitro and in vivo biocompatibility evaluations. Carbohydr Polym. 2011;83(3):1153-1161.
12.     Li M, Li H, Li X, et al. A Bioinspired Alginate-Gum Arabic Hydrogel with Micro-/Nanoscale Structures for Controlled Drug Release in Chronic Wound Healing. ACS Appl Mater Interfaces. 2017;9(27):22160-22175. doi:10.1021/acsami.7b04428
13.     Li H, Duann P, Lin P-H, et al. Modulation of wound healing and scar formation by MG53 protein-mediated cell membrane repair. J Biol Chem. 2015;290(40):24592-24603. doi:10.1074/jbc.M115.680074
14.     Vadakkan K. Acute and sub-acute toxicity study of bacterial signaling inhibitor Solanum torvum root extract in Wister rats. Clin Phytoscience. 2019;5(1):19.
15.     Bakdash A, AL-Mathloum AMK, ElAmin EHA, Taha NMTA, Kumar S, Nasr FA. Single-dose acute toxicity of THJ-2201 designer Cannabis drug: LD 50 and hematological and histological changes in mice. Egypt J Forensic Sci. 2018;8(1):49.
16.     Conlee KM, Stephens ML, Rowan AN, King LA. Carbon dioxide for euthanasia: concerns regarding pain and distress, with special reference to mice and rats. Lab Anim. 2005;39(2):137-161.
17.     Care IA, Committee U. Guidelines for the Use of Carbon Dioxide (CO2) for Rodent Euthanasia. The University of Texas at Austin; 2007.
18.     Everitt JI, Gross EA. Euthanasia and necropsy. In: The Laboratory Rat. Academic Press; 2006:665-678.
19.     OECD. The Organization of Economic Co-Operation and Development (OECD). OECD Guidel Test Chem 420-acute oral toxicity, OECD,. 2001;1001:1-14.
20.     Docie J V. Practical Haemotology. London, J&A Churchill Ltd. 1958:38-42.
21.     Huggett ASG, Nixon DA. Use of glucose oxidase, peroxidase, and o-dianisidine in determination of blood and urinary glucose. Lancet. 1957;270(6991):368-370. doi:10.1016/S0140-6736(57)92595-3
22.     Reitman S, pathology SF-A journal of clinical, 1957  undefined. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. academic.oup.com.
23.     Wybenga DR, Pileggi VJ, Dirstine PH, Di Giorgio J. Direct manual determination of serum total cholesterol with a single stable reagent. Clin Chem. 1970;16(12):980-984.
24.     Lillie RD. Histopathological techniques and practical histochemistry McGraw Hill Book Co. New York. 1965:P176.
25.     Kirtikar KR, Basu BD. Indian medicinal plants vol. 1, International Book Distributors, Dehradun (1987). rat Model Behav Fitoter. 2007;78(2):120-124.
26.     Lee IP, Dixon RL. Factors influencing reproduction and genetic toxic effects on male gonads. Environ Health Perspect. 1978;24:117-127. doi:10.1289/ehp.7824117
27.     HENRY R, CANNON D, WINKELMAN J. Clinical Chemistry Principles and Techniques, 2nd edn (Haper and Row). Hagerstown, MD. 1974:411-421.
28.     Nilakash S, Jonnalagadda G, Chawda MB, Thakur KS, Vahalia MK, Shitut SS. Acute and sub-chronic toxicity (90-Day) study of Swamala (SWA)® in Wistar Rats. Pharm Sci. 2014;20(2):52-60.
29.     Hassanzadeh-Taheri M, Hosseini M, Salimi M, Moodi H, Dorranipour D. Acute and sub-acute oral toxicity evaluation of astragalus hamosus seedpod ethanolic extract in wistar rats. Pharm Sci. 2018;24(1):23-30. doi:10.15171/PS.2018.05
30.     Kluwe WM. Renal function tests as indicators of kidney injury in subacute toxicity studies. Toxicol Appl Pharmacol. 1981;57(3):414-424. doi:10.1016/0041-008X(81)90239-8
31.     OA A-S, TM E-H, AA A-M. Effect of prolonged vigabatrin treatment on hematological and biochemical parameters in plasma, liver and kidney of Swiss albino mice. Sci Pharm. 2002;70(2):135-145. doi:10.3797/scipharm.aut-02-16
32.     Ramesh T, Lee K, Lee HW, Kim SJ. Acute Oral Toxicity Study of Asiasari radix Extract in Mice. Int J Toxicol. 2007;26(3):247-251. doi:10.1080/10915810701352887
33.     Zimmerman HJ, Ishak KG. Hepatic injuries due to drugs and toxins. MacSween al. 1979:335-380.
34.     Akanji MA, Ngaha EO. Effect of Repeated Administration of Berenil on Urinary Enzyme Excretion with Corresponding Tissue Pattern in Rats. Pharmacol Toxicol. 1989;64(3):272-275. doi:10.1111/j.1600-0773.1989.tb00645.x
35.     Adesokan AA, Akanji MA. Effect of administration of aqueous extract of Enantia chlorantha on the activities of some enzymes in the small intestine of rats. Niger Soc Biochem Mol Biol. 2004;18:103-105.
36.     Devlin TM. Textbook of biochemistry: with clinical correlations. 2006.
37.     Crook M. Clinical Chemistry & Metabolic Medicine. Vol 426. Hodder Arnold London; 2006. 

 

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