International Journal of Pharma and Bio Sciences
    ISSN 0975-6299

Int J Pharm Bio Sci Volume 12 issue 4, October - December, Pages:1-7

Diuretic Activity of Hydroalcoholic Extract of Physalis peruviana(Linn.) Leaves in Wistar Rats

Dr. Kundan Singh Bora and Pooja Shah

Physalis is a genus of a perennial, herbaceous or annual plant, which is mostly the local plant of tropical North & South America. Few species of Physalis are widely distributed in warmer parts of world. Some species of Physalis grow in its edible fruits. One or two species occur wildly in India, while three other species are cultivated. Plant Physalis peruviana Linn. (Family: Solanaceae) is one of the important species of genus Physalis found in India, especially leaves & fruits  have been traditionally used as a diuretic, muscle relaxant, in inflammation, for  prevention of tooth decay & abdominal ailments throughout the world. The plant is reported to relieve hypertension, asthma, urinary problem & widely valued for its calming properties. The present study was initiated to investigate the diuretic effect of hydroalcoholic extract of Physalis peruviana (HEPP) in Wistar rats. Hydroalcoholic extract of Physalis peruviana leaves was administered at the dose of 50, 100 & 200 mg/kg,p.o. Hydrochlorothiazide (500mg/kg, p.o.) was used as a positive control in the study. The diuretic effect of the extract was evaluated by measuring the volume of urine, sodium, chloride & potassium content in the urine sample. Urine volume significantly increased at two doses of HEPP extract that is 100 & 200 mg/kg body weight in treated rats. Excretion of chloride, sodium and potassium levels were also increased (77.42 mmol/l, 85.20 mmol/l and 43.6 mmol/l respectively) by HEPP extract (200 ml/kg).The diuretic effect of the hydroalcoholic extract was similar to hydrochlorothiazide at the dose of 200 ml/kg. This study concluded that HEPP produced a notable diuretic effect which appeared to be comparable to that produced by the standard diuretic hydrochlorothiazide. The present study provides a quantitative basis for investigating the use of Physalis peruviana as a diuretic agent.

Keywords: Physalis, Physalis peruviana, Diuretic Activity, Lipschitz Test, Therapeutic Activity, Hydroalcoholic.
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  1. Williams. B., Poulter N.R., Brown M.J., Davis M., Mclnnes G. T., Potter J.F., Sever P.S., Thom S.M. 2004. Brit sh hypertension society guidelines for hypertension management 2004 (BHS-IV): Summary. British medical journal 634-640.
  2. Reyes A.J., Taylor S. H. Diuretics in cardiovascular therapy: The new clinicopharmacological bases that matter. Cardiovascular drugs and therapy 13, Page no. 371-398.
  3. Wright C.I., Van-Buren L., Kroner C.I., Koning M.M.G. Herbal medicine as diuretics: A review of the scientific evidence. Journal of ethnopharmcology 114 (2007). Page no. 1-31. Doi: 10.1016/j.jep.2007.07.023.
  4. Materson B.J., 1983. Insights into intrarenal site and mechanisms of action of diuretic agents. Americal heart journal 106, 188-208.
  5. Puschett, J.B., 1994. Pharmacological classification and renal actions of diuretics. Cardiology 84 (suppl. 2), 4-13.
  6. Goodman and Gilman’s. 12th ed. Page No. 2011. The pharmacological basis of theraputics;677. ISBN: 978-0-07-176939-6. MHID: 0-07-176939-0.
  7. Tripathi KD. Essential of medical pharmacology. 6th ed. Page No; 2008. p. 537-59.
  8. Rang HP, Dale MM, Ritter JM, Flower RJ, Henderson G 2012. Rang And Dale’s Pharmacology. Elsevier ChurchilLivinstone. ISBN-13 978-0-7020-3471-8. Page no. 353-356.
  9. Sayana Suresh Babu, KhanwelkarChitra C, NimmagaddaVenkatRao, DasiJeevan Mani Babu, ChavanVasant R, KutaniAruna, KotagiriKarthik. Evaluation of diuretic activity of alcoholic extract of roots of cissampelospareira in albino rats. J ClinDiagn Res. 2014;8(5). 1-4:HC01-4. doi: 10.7860/JCDR/2014/8192.4350, PMID 24995192.
  10. KasaliMushagalusa F., KadimaNtokamunda Justin, Mpiana T. Pius, NgboluaNyiwg K. TshibanguTshibey D. Assesment of Antidiabetic activity and acute toxicity of leaf extract from Physalisperuviana L. in guinea pig. Asian pacific journal of tropical biomedicine. 2013; 3(11), Page no: 841-846. Doi 10.1016/S2221-1691(13)60166-5.
  11. Fischer G. and Herrera A. 2011. National university of Colombia, Colombia and Almanza P.J., Pedagogical and technological university of Colombia, Colombia. Woodhead Publishing lit. Page no. 377,379,391.
  12. Puente LA, Pinto- CA, Castro ES, Cortes M. Physalisperuviana Linnaeus, the multiple properties of a highly functional fruit: A review. Food Res Int. 2011; 44(7): 1733-1740.
  13. Ellison DH. Ann Intern Med. 114, 1991, 886-894. The physiologic basis of diuretic synergism: its role in treating diuretic resistance. Doi: 10.7326/0003-4819-114-10-886.
  14. Lock olga, Perez Eleucy, Villar Martha, Flores Diana And Rojas Rosario. Bioactive compound from plants used in Peruvian traditional medicine.Natural product communications Vol. 11(3) 2016.
  15. Yen C, Chiu CC, Chang FR, Chen JY, Hwang CC, Hseu YC. 4beta- hydroxywithanolide E from Physalisperuviana inhibit growth of human lung cancer cells through DNA damage, apoptosis and G2/M arrest. BMC Cancer 2010: 10;46. Doi: 10.1186/1471-2407-10-46.
  16. Pardo JM, Fontanilla MR, Ospina LF, Espinosa L. Determining the pharmacological activity of Phsalisperuviana fruit juice on rabbit eyes and fibroblast primary cultures. Invest Ophthalmol Vis Sci 2008; 49(7): 3074-3079.
  17. Pinto Mda S, Ranilla LG, Apostolidis E, Lajolo FM, Genovese MI, Shetty K. Evaluation of antihyperglycemia and antihypertension potential of native peruvian fruits using in vitro models. J Med Food 2009; 12(2): 278-291.
  18. Franco LA, Matiz GE, Calle J, Pinzon R, Ospina LF. Anti- inflammatory activity of extracts and fractions obtained from Physalisperuviana L. calyces. Biomedica 2007; 27(1): 110-115.
  19. Arun M, Asha VV. Preliminary studies on antihepatotoxic effect of Physalisperuviana Linn. (Solanaceae) against carbon tetrachloride induced acute liver injury in rats. J Ethnopharmacol 2007; 111(1): 110-114. 
  20. Javed Ahmad, Farooqui HA, Sageer Ahmad. Trianthemaportulacastrum L., an herbal drug for the cure of edema. J Herbs Species Med Plants. 2008;7(2). Page no:65-70. Doi: 10.1300/J044v07n02_07.
  21. Navdeep Sharma, BanoAnishaDhaliwal S, Harcharan Sharma Vivek. Perspectives and possibilities of Indian species of genus Physalis (L.). EurJournalof Pharm Med Res. 2015;2(2). ISSN 3294-3211. Page no:326-53.  https;//
  22. Olayan-Al EbtisamKhadragy-El, Ahmed Aref M, Mohamed Othman S, Rami Kassab B, Ahmed Moneim Abdel E. 2014. The potential protective effect of Physalisperuviana L. against carbon tetrachloride- induced hepatotoxicity in rats is mediated by suppression of oxidative stress and downregulation of MMP-9 expression. Epub. Page no. 2-8. Doi 10.1155/2014/381413. PMCID: PMC4020166.
  23. Lim TK. Edible Medicinal and non-medicinal plants. Vol. 6. Page no. Dordrecht: Springer Science+Business media; 2013. p. 308. Doi 10.1007/978-90-481-8661-7.
  24. Mohamed Ramadan Fawzy. Bioactive phytochemicals, nutritional value, and functional properties of cape gooseberry (Physalisperuviana): an overview. Food Res Int. 2011;44(7). Page no:1830-6. doi: 10.1016/j.foodres.2010.12.042.
  25. The wealth of India, RAW MATERIALS. Phys Rev E, Page;8(37-40).
  26. Luis Puente A, Munoz-Pinto A Claudia, castro S Eduardo. Cortes misarl. 2010. Physalisperuviana Linnaeus, the multiple properties of a highly functional fruit: A Review, Elsevier. Page no;1734.
  27. Lim TK. Edible medicinal and non-medicinal plants. Dordrecht: Springer science+Business media; 2013. p. 5628. 1-35. Doi 10.1007/978-90-481-8661-7.
  28. Ahmed E, Moneim Abdel Bauomy A. Amira ,Marwa M.S. Diab, M. ShataTarek Mohamed, olayan-al, Khadragy-El F. Manal. The Protective Effect of Physalisperuviana L. Against Cadbium- Induced Neurotoxicity in Rats, Springer, 2014; 160:393.
  29. LanHsuan-Yu Chang Fang-Rong, Mei Pan, Shu-Jing Wu, Su Chen, Shyh Wang, Ming Wu, Yang-Chang Wu. New cytotoxic withanolides from Physalisperuviana. Elsevier, food chemistry. Vol. 2009;116:462-9. Doi: 10.1016/j.foodchem.2009.02.061.
  30. Giraldo H, Luis E, Barrera D, Delgado G, Murcia D, Valdivieso V, Ramirez G. Cytotoxic and immunomodulatory potential activity of Physalisperuviana fruit extracts on cervical cancer (HeLa) and fibroblast. Cells.sagepub. 2017; L929:1-11. PMID: 28719984 .Doi: 10.1177/2156587217718751.
  31. MayuramasNgern-Sang, UiJoungYoun Park Jung-Eun, Kondratyuk P, Simmons, Charles J, Wall Marisa M, Michael Ruf, Lorch Sam E, Ethyn Leong, John Pezzuto M, Chang LengChee. Withanolides derived from Physalisperuviana (Poha) with potential anti-inflammatory activity. Elsevier Bioorg Med ChemLett. 2016:0960-894X. pp1-5.
  32. Cattaneo R, Cavalheiro J, Cossettin N, Tassotti G, Elisa C, Pascoal D, Koefender J, Oliveira C. Antioxidant effect of Physalisperuviana fruit aqueous extract- The antioxidant effect of Physalis. J Agric Sci.1916. 2015; 9760 :137-41. Doi: 10.5539/jas.v7n12p137.
  33. Peter KamauKaranja. ang Ng Zipporah, Gakio Peter, Njeruh M Francis and Thuita John. Anti microbial evaluation and phytochemical screening and aquous and dichloromethane crude extracts of Kenyan Physalisperuviana. 2017(16), PP 101-109. Doi: 10.9790/0853-160509101109. 
  34. Wu Jing-Shu Chang. Pang- Shum, Lin Liang- Doung, Wang- Shyan, MouFeuu- Fwu, Ng Teik- lean. Super critical carbon dioxide extract of Physalisperuviana induced cell cycle arrest and apoptosis in human lung cancer H661 cells. Elsevier. 2009; 1132-8. PMID: 19425186. Doi: 10.1016/j.fct.2009.01.044.
  35. Fokunang C. N., Mushagalusa F. K., Fokunang E. T., Ngoupayo J., Ngameni B., Njinkio L. N., Kadima J. N., Kechia F. A. et al., Phytochemical and zootechnical study of Physalisperuviana L. leaves exposure to streptozotocin induced diabetic rats. Vol.9(8), Page no. 123-130. Doi: 10.5897/JPP2016.0418.
  36. Karim N, Khan H, Ayub B, Abdel-Halim H and Gavande N. Anxiolytic potential of Natural Flavonoids. SM J Steroids Horm. 2018;1(1):1001.
  37. Wu J, Tsai J, Chang S, Lin D, Wang S, Huang S, Ng L. Supercritical carbon dioxide extract exhibits enhanced antioxidant and anti-inflammatory activities of Physalisperuviana. ScienceDirect. 108. 2006. p. 407-13. Doi : 10.1016/j.jep.2006.05.027.
  38. Vogel GH. Drug discovery and evaluation: pharmacological assays. Springer. ISBN 3-540-42396-6. Page no. 2002;64:323-4. RM301.25.V64 2002.
  39. Charles Craig R, Robert Stitzel E. Modern pharmacology with clinical applications. 5th ed. Pp. 357-9.
  40. Zhang Wen-Na, Tong Wang-Yu. Chemical constituents and biological activities of plants from the genus Physalis. ChemBiodivers. 2016;13(1):48-65. doi: 10.1002/cbdv.201400435, PMID 26765352.
  41. Melka AE, Makonnen E, Debella A, Fekadu N, Bekesho G. Evaluation of diuretic activity of methanol crude extract of Thymus serrulatus leaves and its solvent fraction in mice. Biol Med. 2016. doi: 10.4172/0974-8369. Page no. 2-6.
  42. Fischer G, Herrera H 2011. Cape gooseberry (Physalisperuviana L.). WoodheadPublishingLtd. Page no. 375-391.
  43. Ramkumar G G. et al. Diuretic herbs on siddha system of medicine - a review, International Journal of Research in Pharmaceutical and Nano Sciences, 3(1), 2014, 43-49.
  44. Mishra Snigdha et al., Sch. J. App. Med. Sci., 2013; 1(3):209-214
  45. Wright CI, Van-Buren L, Kroner CI, Koning MMG (2007) Herbal medicines as diuretics: a review of the scientific evidence. Journal of Ethnopharmacology 114: 1-31. doi:10.1016/j.jep.2007.07.023
  46. Kau S. T., Keddie J. R. and Andrews D. A method for screening diuretic agents in the rat.
  47. Tao-Sheng Fang, Bo Li, Kai-Ji Liu. Two new withanolides from Physalisperuviana. HelvChimActa. 2009;92(7). Page no:1304-8. doi: 10.1002/hlca.200900005.
  48. Jyothibasu T, Vinkata K, Thalla S, Narasimha C. Diuretic activity of methanolic extract of Physalisminima leaves. Der Pharmacia Lettre, 2012,4(6):1832-1834.
  49. Yu-Zhou Guan, Si-Ming Shan, Wei Zhang, Guang-JianLuo, Ling-Yi Kong. Withanolides from Physalisminima and their inhibitory effects on nitric oxide production. Steroids. 2014;82. Page no:38-43. doi: 10.1016/j.steroids.2014.01.004.
  50. Beltagi S. H., Mohamed H., Safwat G., Gamal M., Megahed B. Chemical composition and biological activity of Physalisperuviana L.
  51. Nayak B.S., Ellaiah P., Dinda S. C., Moharana B. P., Khadanga M. and Nayal S. Diuretic activity of flavonoid compound isolated from Gmelinaarborea fruits extract.
  52. Tariq N., Waheed A., Majeed M. I., Hanif M., Rehman R., Eddouks M. Indian Globe Thistle.





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