International Journal of Pharma and Bio Sciences
 
 
    ISSN 0975-6299
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ORIGINAL RESEARCH ARTICLE
Int J Pharm Bio Sci Volume 12 issue 4, October - December, Pages:34-39

Antiproliferative Activity of Methanol Extract of Stachytarpheta Urticifolia (Salisb.) Sims Leaf on Human Cancer Four Different Cell Lines (Funded Work)

Sreelatha Regalagadda and Murali Mohan Challa
DOI: http://dx.doi.org/10.22376/ijpbs.2021.12.4.p34-39
Abstract:

Cancer represents one of the most serious health problems and major causes of death around the world. Many anticancer drugs in clinical use today are natural products or derived from natural sources. Stachytarpheta urticifolia is a herbal medicine widely distributed in many parts of the world. The antiproliferative activities of the methanolic extract of Stachytarpheta urticifolia   leaves collected from Damuku rai village of Araku Valley near Ananthagiri Mandal in Visakhapatnam District, against HT-29, A-431, A-549 and Hep-G2 cell lines were investigated by using 3-(4,5-dimethyl thiazol-2-yl)-2, 5-diphenyl Tetrazolium Bromide (MTT) assay. The extract showed a strong antiproliferative activity against all cell lines with IC50 values of 20.89, 27.56, 38.45 and 49.65 at 250 μg/ml, respectively. Flow cytometry results showed that the extract arrested the cell cycle at S phase and the increase in the percentage of caspase 3 activity in HT-29 and the A-431 cells by 129.41% and 112.8 % respectively  suggested that the extract could induce cell apoptosis by a caspase mediated pathway. These results demonstrated that the methanolic extract of Stachytarpheta urticifolia leaves collected from Damuku rai village has comparable strong antiproliferative activity. The research demonstrated that the Stachytarpheta urticifolia extracts from different parts of the plant had shown antiproliferative and induced Caspase-3 activity against the human cancer cell line. The findings of this study indicate that there is some justification for the use of Stachytarpheta urticifolia as anti-proliferative activity of Caspase-3 activity induced cell apoptosis by caspase mediated pathway.

Keywords: Antiproliferative activity, MTT Assay, Flow cytometry, Stachytarpheta urticifolia, HT-29, A-431, A-549 and Hep-G2
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REFERENCES

 

  1. Ochwang’I DO, Kimwele CN, Oduma JA, Gathumbi PK, Mbaria JM, Kiama SG. Medicinal plants used in treatment and management of cancer in Kakamega County Kenya. Journal of Ethnopharmacology. 2014 Feb12; 151(3):1040–1055.
  2. Graham JG, Quinn ML, Fabricant DS, Farnsworth NR. Plants used against cancer-an extension of the work of Jonathan Hartwell. JEthnopharmacol. 2000 Dec1; 73(3): 347-377.
  3. Shoeb M. Anticancer agents from medicinal plants. Bangladesh J Pharmacol. 2006 Nov 29; 1(2): 35-41.
  4. Cragg GM, Newman DJ. Natural product scaffolds lead to drugs. Future Med Chem. 2009 Nov 1; 1(8): 1415-1427.
  5. World Health Organization, “World Health Statistics Annual 1996,” World Health Organization, Geneva, 1998.
  6. Paolo V, Kirsti HP. Air pollution and cancer: biomarker studies in human populations. Carcinogenesis. 2005 Nov; 26(11): 1846-1855.
  7. Gerald NW, Stephen SH, James SF, Allan HC, Lawrence AL. Environmental and chemical carcinogenesis. Seminars in Cancer Biology. 2004; 14(6): 473-486.
  8. Gulam W, Haseeb A. Reactive oxygen species: role in the development of cancer and various chronic conditions. Journal of Carcinogenesis. 2006 ;( 14): 1-8.
  9. World Health Organization Cancer Statistics (Search), 2013; Accessed on Dec12. http://www.who.int/mediacentre/factsheets/fs297/en/.
  10.  Rajesh S, Patric Raja D, Rathi JM and. Sahayaraj K.Biosynthesis of silver nanoparticles using Ulva fasciata (Delile) ethyl acetate extract and its activity against Xanthomonas campestris pv. Malvacearum. JBiopest. 2012 April 5; (Supplementary): 119-128.
  11. Indap MA, Radhika S, Motiwale L, Rao KVK. Quercetin: antitumor activity and pharmacological manifestations for increased therapeutic gains. Indian J Pharm Sci. 2006 July 20; 68: 465-469
  12. Pan L, Chai H, Kinghorn AD. The continuing search for antitumor agents from higher plants. Phytochem Lett .2010 March12; 3(1): 1-8.
  13. Li WL, Zheng HC, Bukuru J, Kimpe ND. Natural medicines used in the traditional Chinese medical system for therapy of diabetes mellitus. Journal of Ethnopharmacology. 2004 May; 92 (1): 1–21.
  14. Frank EK, Guy TC. The evolving role of natural products in drug discovery. Nature Reviews Drug Discovery. 2005 March; 4:206-220.
  15. Jie, P. and Ning, J. Oral cavity infection: an adverse effect after the treatment of oral cancer in aged Individuals. Journal of Applied Oral Science. 2014 Dec 12; 22(4):  261-7.
  16. Cragg GM, Grothaus PG, Newman DJ. Impact of natural products on developing new anti-cancer agents. Chem Rev 2009 May 7; 109(7): 3012-3043.
  17. Bhushan P, Ashok DBV, Mukund C. Ayurveda and natural products drug discovery. Current Science. 2004 March; 86 (6):789-799.
  18. Sivagurunathan S, Natalie AE, Dominic TM, George WS, Yue YS, Robert ZO. Dietary curcumin inhibits chemotherapy-induced apoptosis in models of human breast cancer. Cancer Research. 2002 July 1;  62(13): 3868–3875,
  19. Ricky AS, Stephanie AE, Sharon LP, Darren NC, Aisha S,1 Heather RH, Timothy HM, Bruno M, David H, Simon MP, Munir P, Andreas JG, William PS. Phase I clinical trial of oral curcumin: biomarkers of systemic activity and compliance. Clinical Cancer Research. 2004 Oct 15; 10 (20): 6847–6854.
  20. Cordell GA, Beecher CW, Pezzuto JM. Can ethnopharmacology contribute to the development of new anticancer drugs? J Ethnopharmacol. 1991 April; 32(1-3): 117-133.
  21. Kintzios SE. Terrestrial plant derived anticancer agents and plant species used in anticancer research. Crit Rev Plant Sci. 2006 May; 25(2): 79-113.
  22. Moyo B, Mukanganyama S. "Antiproliferative Activity of T. welwitschii Extract on Jurkat T Cells In Vitro", BioMed Research International. 2015 Sep 3; 1-10.
  23. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 1983 Dec16; 65: 55-63.
  24. Al-Abd AM, Al-Abbasi FA, Asaad GF, Abel Naim AB. Didox potentiates the cytotoxic profile of doxorubicin and protects from its cardiotoxicity. Eur J Pharmacol. 2013 Oct 15; 718(1-3): 361-9.
  25. Mehta RG, Murillo G, Naithani R, Peng X. Cancer chemoprevention by natural products: how far have we come? Pharmacol Res.  2010 March 18; 27: 950-961.
  26. Soobrattee MA, Bahorun T, Aruoma OI. Chemopreventive actions of polyphenolic compounds in cancer. Biofactors.  2006 Jan 01; 27: 19-35.
  27. Suffness M, Pezzuto JM. Assays related to cancer drug discovery, In: Hostettmann, K. (Ed). Methods Plant Biochemistry: Assays for Bioactivity. London. Academic Press. 1990; 6: 71-133.
  28. Ahmed I, Islam M, Arshad W, Mannan A, Ahmad W, Mirza B. High quality plant DNA extraction for PCR: an easy approach. J Appl Genet. 2009 June; 50(2):105-107.
  29. Regalagadda S, Challa MM, Kasturi APK, Edavana SK, Santosh KS. Phytochemical screening, total phenol content and antioxidant activity of different parts of Stachytarpheta urticifolia (Salisb) Sims. JPR:BioMedRx: An International Journal. 2013 June 26; 1(7):  718-723.
  30. Ozturk S, Ercisli S. Antibacterial activity and chemical constitutions of Ziziphora clinopodioides. Food Control. 2007; 18:535-540.
  31. Earnshaw WC, Martins LM, Kaufmann SH. Mammalian caspases: structure, activation, substrates, and functions during apoptosis. Ann Rev Biochem. 1999 Feb; 68:383-424.
  32. Hengartner MO The biochemistry of apoptosis. Nature. 2000 Oct 12; 407:770-6.
  33. Kim R, Tanabe K, Uchida Y, Emi M, Inoue H, Toge T. Current status of the molecular mechanisms of anticancer drug-induced apoptosis. The Contribution of molecular- level analysis to Cancer Chemotherapy.Cancer Chemother Pharmacol. 2002 Nov; 50:343-52.

 

 

 

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