Silver nanopartilcles are ecofriendly in nature. The aim of the current research is to determine, optimizes, biosynthesis, characterize silver nanoparticles and its antimicrobial determination.  The present study reveals a novelty in extracellular eco friendly-biosynthesised AgNPs using the pod coat aqueous extract of Corchorus olitorius as a reducing agent. The biosynthesised   AgNPs  were characterised by using spectral analysis UV-visible spectroscopymethods, Transmission Electron Microscopy, X-ray diffraction studies, and energy dispersive X-ray. Antimicrobial biograms of AgNPs was recorded by cup plate technique. The manifestation, dimension and contour of the silver nanoparticles were determined by UV-visible spectroscopy and transmission electron microscopy. The X-ray diffraction analysis indicates that particles are crystalline in nature. Fourier transform infrared spectroscopy analysis showed that the nanoparticles are enclosed with bio-moieties on their shell. The current examination records the bio functionalized silver nanoparticles thus shown commendable antimicrobial properties. The current formula implicated is eco-friendly, effortless and hence high range production of the same can be measured for using them in numerous pharmaceuticals.

References

1. Shivakumar Singh P, Vidyasagar GM. Biosynthesis, Characterization, and Antidermatophytic Activity of Silver Nanoparticles Using Raamphal Plant ( Annonareticulata ) Aqueous Leaves Extract. Indian J Mater Sci. 2014;2014:1-5. doi: 10.1155/2014/412452.
2. Feymen F. There^’s plenty of room at the bottom, Science. 1991;254:1300-1.
3. Bigall NC, Eychmüller A. Synthesis of noble metal nanoparticles and their non-ordered superstructures. Philos Trans A Math PhysEng Sci. 2010;368(1915):1385-404. doi: 10.1098/rsta.2009.0274, PMID 20156829.
4. Balantrapu K, Goia DV. Silver nanoparticles for printable electronics and biological applications. J Mater Res. 2009;24(9):2828-36. doi: 10.1557/jmr.2009.0336.
5. Tripathi RM, Saxena A, Gupta N, Kapoor H, Singh RP. High antibacterial activity of silver nanoballs against E.coli MTCC 1302, S.typhimurium MTCC 1254, B.subtilis MTCC 1133 and P.aeruginosa MTCC 2295. Digest J NANO-Mater Biostructures. 2010;5(2):323-30.
6. Patakfalvi R, Dékány I. Preparation of silver nanoparticles in liquid crystalline systems. Colloid Polym Sci. 2002;280(5):461-70. doi: 10.1007/s00396-001-0629-0.
7. Rodríguez-Sánchez L, Blanco MC, López-Quintela MA. Electrochemical synthesis of silver nanoparticles. J PhysChem B. 2000;104(41):9683-8. doi: 10.1021/jp001761r.
8. Taleb A, Petit C, Pileni MP. Optical properties of self-assembled 2D and 3D superlattices of silver nanoparticles. J PhysChem B. 1998;102(12):2214-20. doi: 10.1021/jp972807s.
9. Crooks RM, Lemon BI, Sun L, Yeung LK, Zhao M. Dendrimer-Encapsulated Metals and Semiconductors: Synthesis, Characterization, and Applications. Top Curr Chem. 2001;212:81-135. doi: 10.1007/3-540-44924-8_3.
10. Bhumkar DR, Joshi HM, Sastry M, Pokharkar VB. Chitosan reduced gold nanoparticles as novel carriers for transmucosal delivery of insulin. Pharm Res. 2007;24(8):1415-26. doi: 10.1007/s11095-007-9257-9, PMID 17380266.
11. Poovi G, Dhanaleks UM, Narayanan N, Neelakanta P. Preparation and characterization of repaglinide loaded chitosan polymeric nanoparticles. Research J of Nanoscience and Nanotechnology. 2011;1(1):12-24. doi: 10.3923/rjnn.2011.12.24.
12. Robenek H. Colloidal gold: Principles, methods, and applications vols. I and II (vol. III in preparation) Edited by M. A. Hayat Academic Press, Inc., New York, 1989 ISBN 0-12-333927-8, Vol. I, 536 pages ISBN 0-12-333928-6, Vol. II, 484 pages. Scanning. 1990;12(4):244-. doi: 10.1002/sca.4950120410.
13. Gittins DI, Bethell D, Nichols RJ, Schiffrin DJ. Diode-like electron transfer across nanostructured films containing a redox ligand. J Mater Chem. 2000;10(1):79-83. doi: 10.1039/a902960e. Gittins DI, Bethell D, Nichols RJ, Schiffrin DJ. Diode-like electron transfer across nanostructured films containing a redox ligand. J Mater Chem. 2000;10(1):79-83. doi: 10.1039/A902960E.
14. Dai J, Bruening ML. Catalytic nanoparticles formed by reduction of metal ions in multilayered polyelectrolyte films. Nano Lett. 2002;2(5):497-501. doi: 10.1021/nl025547l.
15. Li X, Jia Y, Cao A. Tailored single-walled carbon nanotube--CdS nanoparticle hybrids for tunableoptoelectronic devices. ACS Nano. 2010;4(1):506-12. doi: 10.1021/nn901757s, PMID 20041712.
16. Savithramm N, Rao ML, Devi PS. Evaluation of Antibacterial Efficacy of Biologically Synthesized Silver Nanoparticles using Stem Barks of Boswelliaovalifoliolata Bal. and Henry and ShoreatumbuggaiaRoxb J of Biological Sciences. 2011;11(1):39-45. doi: 10.3923/jbs.2011.39.45.
17. Ramgopal M, Saisushma C, HamadAtti I, M. Alhasin A. A Facile Green Synthesis of Silver Nanoparticles using Soap Nuts. Research J of Microbiology. 2011;6(5):432-8. doi: 10.3923/jm.2011.432.438.
18. Sinha S, Pan I, Chanda P, Sen SK. Nanoparticles fabrication using ambient biological resources. J ApplBiol Sci. 2009;19:1113-30.
19. Policepatel SS, Manikrao VG. Ethno medicinal plants used in the treatment of skin diseases in Hyderabad Karnataka region, Karnataka, India. Asian Pac J Trop Biomed. 2013;3(11):882-6. doi: 10.1016/S2221-1691(13)60173-2.
20. Kannan N, Subbalaxmi S. Green synthesis of silver nanoparticles using Bacillus subtillus IA751 and its antimicrobial activity. Research J of Nanoscience and Nanotechnology. 2011;1(2):87-94. doi: 10.3923/rjnn.2011.87.94.
21. Ramadevi D, Ganapaty S. Phytochemical Examination of Corchorusolitorius roots. PHARMATUTOR-ART-1171, 1998. Reference id: PHARMATUTOR-ART-1171.
22. Cochorus L, Germplasm Resources information Network, United states Department of Agriculture. Vol. 03. p. 13 (2009); 2003. Retrieved. Available from: https://www.ars-grin.gov/ [cited 30/10/2020].
23. Gupta M, Mazumder UK, Pal DK, Bhattacharya S. Onset of puberty and ovarian steroidogenesis following adminstration of methanolic extract of CuscutareflexaRoxb. stem and Corchorusolitorius Linn. Seed in mice. J Ethnopharmacol. 2003;89(1):55-9. doi: 10.1016/s0378-8741(03)00262-9, PMID 14522432.
24. Pal DK, Mandal M, Senthilkumar GP, Padhiari A. Antibacterial activity of Cuscutareflexa stem and Corchorusolitorius seed. Fitoterapia. 2006;77(7-8):589-91. doi: 10.1016/j.fitote.2006.06.015, PMID 16890386.
25. Nakamura T, Goda Y, Sakai S, Kondo K, Akiyama H, Toyoda M. Cardenolide glycosides from seeds of Corchorusolitorius. Phytochemistry. 1998;49(7):2097-101. doi: 10.1016/s0031-9422(98)00421-x. PMID9883596.
26. Duvold T, Jørgensen A, Andersen NR, Henriksen AS, Dahl Sørensen M, Björkling F. 17S,20S-Methanofusidic acid, a new potent semi-synthetic fusidane antibiotic. Bioorg Med ChemLett. 2002;12(24):3569-72. doi: 10.1016/s0960-894x(02)00797-7. PMID 12443778.
27. Sun Xia, Sun Y. Shape-controlled synthesis of gold and silver nanoparticles, Science. 2002; (298)5601; 2176-2179. DOI: 10.1126/science.1077229
28. Prasad R, Swamy VS. Antibacterial activity of silver nanoparticles synthesized by bark extract of Syzygiumcumini. Journal of Nanoparticles. 2013; 6-12. Article ID 431218, https://doi.org/10.1155/2013/431218
29. Smitha SL, Philip D, Gopchandran KG. Green synthesis of gold nanoparticles using Cinnamomumzeylanicum leaf broth,” SpectrochimicaActa - Part A: Molecular and Biomolecular Spectroscopy. 2009; (74)3, 735–739. doi: 10.1016/j.saa.2009.08.007.
30. Anandalakshmi K, Venugobal J, Ramasamy V. Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity. ApplNanosci. 2016; (6)399–408. DOI 10.1007/s13204-015-0449-z
31. Shankar SS, Ahmad A, Sastry M. Geranium leaf assisted biosynthesis of silver nanoparticles. BiotechnolProg. 2003;19(6):1627-31. doi: 10.1021/bp034070w, PMID 14656132.
32. Anandalakshmi K, Venugobal J, Ramasamy V. Characterization of silver nanoparticles by green synthesis method using Pedalium murex leaf extract and their antibacterial activity. ApplNanosci. 2016;(6) 399–408.
33. Bahareh Khodashenas, Hamid RezaGhorbani. Synthesis of silver nanoparticles with different shapes.Arabian Journal of Chemistry. 2019; (12)8,1823-1838. https://doi.org/10.1016/j.arabjc.2014.12.014
34. Hussain SAK. PalIncorporation of nanocrystalline silver on carbon nanotubes by electrodeposition technique Mater.Lett. 2008; (62) 1874-1877.  doi: 10.3390/ma12182889
35. Gurunathan S, Kalishwarlal R, Aidyanathan V, Deepak S, Muniyandi J. Purification and characterization of silver nanoparticles using Escherichia coli. Colloids Surf. B.2009; (74)  328-335. DOI: 10.1016/j.colsurfb.2009.07.048.