International Journal of Pharma and Bio Sciences
    ISSN 0975-6299

Int J Pharm Bio Sci Volume 13 Issue 4, October - December, Pages:1-8

Rapid In Vitro Plant Regeneration From Nodal Explant of Mucuna Gigantea-An Endangered Medicinal Legume

Sanjay K. Madkami, Arpita Moharana, Ramesh C. Mishra and Durga P. Barik

Mucuna gigantea (Willd.) DC. (Fabaceae) is an underutilized endangered medicinal legume distributed in tropical Asia, Japan, Australia, the Pacific islands and Africa. Almost all parts of the plant have been found to contain L-3,4-dihydroxy phenylalanine (L-DOPA), a non-protein amino acid that works as a precursor for the neurotransmitter dopamine. Keeping in view,   efforts have been made to develop an efficient, quick and large scale propagation protocol using mature nodal segments of M. gigantea. The aim of the research was to create a quick in vitro regeneration of shoot and root optimisation of the cytokinin and auxin are required. Several cytokinins, 6-benzyladenine (BA), kinetin (Kin), meta-Topolin (mT), Thidiazorone (TDZ) and auxins, Indole-3-butyric acids (IBA), α-Napthalene acetic acids (NAA) singularly or in combinations were supplemented for in vitro shoot multiplication. Among them, BA was efficient in producing several shoots at an ideal dosage of 2.0 mg/l and IBA 0.5 mg/l for roots. The effectiveness of shoot regeneration was also controlled by the strength and NaCl concentration in the basal media. When the NaCl concentration was lowered, the frequency of shoot regeneration tends to increase up to a certain limit. On MS medium supplemented with 2.0 mg/l BA and 0.5 mg/l NAA, the highest response 83.3% with peak number of multiple shoots c.a. 9.1 and maximum average shoot length 5.7 cm were standardised at four sub-cultures. For up-scaling of shoots, in vitro nodal segments were harvested, cultured and highest shoot multiplication was observed on MS+1.0 mg/l BA. In vitro shoots removed from proliferating shoot cultures were best encouraged to root on ½ MS medium supplemented with an ideal concentration of 0.5 mg/l IBA with an average 3.1 root numbers with an average 68% survival rate, the in vitro-raised plantlets with well-developed shoots and roots were successfully established in earthen pots containing garden soil and nurtured in a greenhouse. The finding of this study is the first to show in vitro regeneration of Mucuna gigantea. This plant regeneration procedure, would be valuable for ex situ conservation, reintroduction in wild habitat and commercial application in the pharmaceutical industries, which will change the underutilized to utilized legume.


Keywords: Fabaceae; Mucuna gigantea; Mature nodal Explants; Plant Regeneration; Tissue Culture.
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