10.22376/ijpbs.2019.10.1.p1-12 Volume 2 Issue 4 2011 (October - December) Bacteriocin producing lessThan i greaterThan Lactobacillus reuteri lessThan /i greaterThan strain isolated from human breast milk, showed broad spectrum of antimicrobial activity against clinical pathogens. Maximum bacteriocin production was observed at 37 lessThan sup greaterThan 0 lessThan /sup greaterThan C and pH 7. In addition of enzymes such as α-amylase, proteinase K, Lysozyme, methanol, chloroform, ethanol, acetone and benzene were strongly inhibited bacteriocin production. The bacteriocin has purified by ammonium sulphate precipitation, dialysis and Ion exchange chromatography. Biochemically it was pure protein and the molecular weight was 10kDa. The study revealed that bacteriocin producing lessThan i greaterThan Lactobacillus reuteri lessThan /i greaterThan may be used as probiotic to inhibit pathogens.  Ilayaraja.R And Radhamadhavan Probiotics, Antimicrobial activity, Arbitory unit, Medicine. 552-560