10.22376/ijpbs.2019.10.1.p1-12 Volume 2 Issue 4 2011 (October - December) Antiurolithic effect of aqueous extract of Terminalia chebula, Glycyrrhiza glabra, Nelumbo nucifera, Zingiber officinale, Hemidesmus indicus, Myristica fragrans and Citrus aurantofolia. was tested. 24 albino wistar rats were divided into 4 groups of 6 each. Group-I is control. Groups-II, III and IV were fed 0.75% ethylene glycol by gastric lavage and 1 gm Ammonium chloride in drinking water . Groups-III and IV received aqueous extract of polyherbal formulation at a dose of 1mg/kg body weight and 2mg/kg body weight. . Statistical Analysis: One way of Analysis of Variance was used to test the statistical significance. Results.In urolithiatic animals,Calcium and Oxalate excretion was grossly increased. Groups III & IV showed weight gain, decreased calcium and oxalate excretion, increased levels of urinary volume , pH and stone inhibitors. Histopathological examination of renal tissues confirms this result. Conclusion: The polyherbal formulation used in this study has preventive effect in the formation of renal calculi. L.Akila, P. Ashok Kumar And P.Nirmala Urolithiasis; Poly herbal formulation; Ethylene glycol; Ammonium chloride, urinary stone inhibitors. 07-24