10.22376/ijpbs.2019.10.1.p1-12 Volume 2 Issue 4 2011 (October - December) HPTLC method for simultaneous determination of β-amyrin and stigmasterol in lessThan i greaterThan Putranjiva roxburghii lessThan /i greaterThan wall. has been developed and validated. The analytes were separated on silica gel 60F lessThan sub greaterThan 254 lessThan /sub greaterThan HPTLC plates with n - hexane: chloroform: methanol (3: 6.5:0.5 v/v/v) as mobile phase after chamber saturation for 10 min. The development distance was 80mm. The derivatization was done by using anisaldehyde – sulphuric acid reagent. Detection and quantification were performed by densitometry, with a tungsten lamp, at 580 nm. The response to β-amyrin and stigmasterol was linear in the concentration range 0.045 to 0.360 μg per band and 0.041 to 0.328 μg per band respectively. The validated method was used for quantitative analysis of β-amyrin and stigmasterol in lessThan i greaterThan Putranjiva roxburghii lessThan /i greaterThan wall. and can be used for routine quality-control analysis of leaf powder of lessThan i greaterThan Putranjiva roxburghii lessThan /i greaterThan wall. Madhavi Badole, Dr.Vidya Dighe And Gaauree Charegaonkar Putranjiva roxburghii wall., β-amyrin, stigmasterol, HPTLC, Densitometry. 346-352