10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 1 2012 (January - March) lessThan i greaterThan Thermoactinomyces thalpophilus lessThan /i greaterThan (Gene Bank accession number EF 397000) isolated from the waters of a thermal spring situated in Western Ghats, Maharashtra, India was found to be a potent producer of thermolysin-like protease (TLP). TLP was capable of bringing about condensation/ biotransformation of two amino acids, viz. Z-L-aspartic acid and L-phenylalanine methyl ester into aspartame, which is an artificial, non-saccharide sweetener used as a sugar substitute in some foods and beverages. The maximum yield 181 U/ml/min was obtained at 55 lessThan sup greaterThan o lessThan /sup greaterThan C in glucose yeast extract medium(pH 7.2) containing CaCl lessThan sub greaterThan 2 lessThan /sub greaterThan ( 0.2 % w/v) with an inoculum size of 0.5% v/v after 18 hours incubation under shake-culture conditions (130 rpm) D.R Majumder And P.P Kanekar TLP, biotransformation, aspartame, Thermoactinomyces thalpophilus., Thermolysin 610-627