10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 2 2012 (April - June) lessThan i greaterThan Psidium guajava lessThan /i greaterThan L is commonly known as guava in English. The present investigation focuses on the toothache activity. The investigation is based upon the analgesic, anti-inflammatory and anti-microbial activity of water extract of lessThan i greaterThan Psidium guajava lessThan /i greaterThan L. The study assessed the different solvent extracts of lessThan i greaterThan Psidium guajava lessThan /i greaterThan leaf for thin layer chromatography (TLC) and also evaluated the analgesic activity by Hot plate and acetic acid induced writhing, anti-inflammatory action by lessThan i greaterThan Invitro lessThan /i greaterThan HRBC membrane stabilization method and antimicrobial activity by Disc diffusion method, Agar well diffusion method and Minimum inhibitory concentrations (MIC) in animal model. The present preliminary study demonstrated marked toothache activity of lessThan i greaterThan Psidium guajava lessThan /i greaterThan leaf. These results support the notion that plant flavonoids and extracts may have many roles as pharmaceuticals. S. Jayakumari, J. Anbu, V. Ravichandiran, S.Nithya, Ashwini Anjana, And D. Sudharani Psidium guajava L, analgesic, anti-inflammatory, anti-microbial, Toothache 238-249