10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 4 2012(October - December) Nodal segments from six years old plants of lessThan i greaterThan Bauhinia variegata lessThan /i greaterThan L. were cultured on Murashige and Skoog lessThan sup greaterThan , lessThan /sup greaterThan s (MS) medium supplemented with different concentration and combinations of BAP and IBA. Explants culture in MS basal medium supplemented with 5mg/1 BAP showed highest rate of shoot multiplication. When lessThan i greaterThan in vitro lessThan /i greaterThan shoots were inoculated on the MS basal medium supplemented with IBA 2mg/l, profussed rooting was observed. lessThan i greaterThan Bauhinia variegata lessThan /i greaterThan , commonly known as Kachnar is considered as medicinal plant in Nepal and India. The ethanolic extract of this plant was found to have antimicrobial activity against lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan (MTCC8), lessThan i greaterThan E coli lessThan /i greaterThan (MTCC1), lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan (MTCC98), lessThan i greaterThan Salmonella typhi lessThan /i greaterThan (MTCC737). The largest zone of inhibition (16 mm) was found against lessThan i greaterThan B. subtilis lessThan /i greaterThan . The extract was found to be more effective against gram- positive than gram-negative bacteria. MADHU KUMARI Bahuina variegata, nodal segment culture, In vitro multiplication, multiple shoot induction, plantlet regeneration, antimicrobial activity. 43-50