10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 4 2012(October - December) Multiple shoots were induced in cotyledon and leaf explants of lessThan i greaterThan Solanum surattense lessThan /i greaterThan Burm. f., a medicinally important Solanaceae member. Cotyledon and leaf explants isolated from axenic seedlings (3-5 weeks old) were cultured on the Murashige and Skoog medium supplemented with different concentrations of Thidiazuron (TDZ) (2.27, 2.72, 3.17, 3.63, 4.08 & 4.54 µM) alone or in combination with Indole 3- acetic acid (IAA) (0.28 µM). Maximum number of shoots (72.5±1.70 & 68.1±1.12) were induced directly (without callus) and maximum shoot length (8.25± 0.31 & 7.03 ± 0.53) was observed in cotyledon and leaf explants, respectively. Microshoots obtained in the above treatments were rooted on MS + Indole lessThan b greaterThan - lessThan /b greaterThan 3- butyric acid (IBA) (4.92 µM). Complete plantlets (2n=24) were transferred to research field and 80-90 % of these plantlets survived. Plantlets regenerated from cotyledon and leaf explants did not show any morphological and cytological variations and they flowered normally and set fruit. RAVI CHITHAKARI, AVINASH KUMAR S, GULAB KHAN R, PRASAD B AND CHRISTOPHER REUBEN T Murashige and Skoog medium, Cotyledon, Thidiazuron, Direct organogenesis. 147-151