10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 4 2012(October - December) A protocol for lessThan i greaterThan in vitro lessThan /i greaterThan micropropagation was developed for lessThan i greaterThan Taverniera cuneifolia lessThan /i greaterThan (Roth) Arn; a substitute for commercial liqorice- lessThan i greaterThan Glycyrrhiza glabra lessThan /i greaterThan , using nodal explants derived from lessThan i greaterThan in vitro lessThan /i greaterThan raised 30 days old seedlings. The excised axillary tips were cultured on Murashige and Skoog (MS) medium containing BA, Kinetin alone or in combination with AgNo lessThan sub greaterThan 3 lessThan /sub greaterThan for multiple shoot induction. The best condition for shoot growth was with 2 mgl lessThan sup greaterThan -1 lessThan /sup greaterThan BA and 5.07 mgl lessThan sup greaterThan -1 lessThan /sup greaterThan AgNo lessThan sub greaterThan 3 lessThan /sub greaterThan in MS medium. Averages of 5.36 shoots were obtained per explant after 30 days of culture. The rooting of lessThan i greaterThan in vitro lessThan /i greaterThan developed shoots (4~6 cm length) was best in MS medium containing 3 % sucrose and 2 mgl lessThan sup greaterThan -1 lessThan /sup greaterThan NAA. Micropropagated plants were successfully acclimatized (60 %) within 6 weeks after rooting. The standardized protocol reported in this study may help in the conservation of this plant which is currently exploited from nature. VIJAY C. JAMDHADE, SHRIVARDHAN V. BALKHANDE AND BABASAHEB S SURWASE Axillary bud multiplication, Glycyrrhiza glabra, Medicinal plant, Taverniera cuneifolia Abbreviations : BA : N 6-benzyladenine, Kn : Kinetin , AgNo3 : Silver nitrate , NAA : Naphthalene acetic acid , IAA : Indole-3-acetic acid, EMS: Ethyl methane sulfonate. 204-212