10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 4 2012(October - December) The study is attempted to investigate the production of potent lipase from groundnut oil mill fungi. The fungi was isolated and identified as lessThan i greaterThan Trichoderma sp lessThan /i greaterThan . Lipolytic activity of lessThan i greaterThan Trichoderma sp. lessThan /i greaterThan was preliminarily confirmed by Rhodamine B method, lipase production was carried out using mineral medium. The extracellular protein concentration and lipase enzyme activity (7.83U/ml) was maximum at 4 lessThan sup greaterThan th lessThan /sup greaterThan day of incubation period. lessThan i greaterThan Trichoderma sp lessThan /i greaterThan . was studied by using different carbon, oil and nitrogen sources. It was found that carbon, oil and nitrogen sources like glucose, groundnut oil and sodium nitrate were shown the maximum lipase activity. From the ANOVA analysis, the effect of different sources on lipase production was more significant.  A.PRAVEEN KUMAR, K.JAYA KUMAR AND G.NARASIMHA Trichoderma sps, Lipase enzyme activity, different sources, production 275-280