DIOXYGENASE GENE OF PAHs DEGRADING A BACILLUS STRAIN ISOLATED FROM MARINE INDONESIAN ENVIRONMENT AND ITS BIOSURFACTANT PRODUCTION ABILITY

10.22376/ijpbs.2019.10.1.p1-12

Volume 3 Issue 4 2012(October - December) DIOXYGENASE GENE OF PAHs DEGRADING A BACILLUS STRAIN ISOLATED FROM MARINE INDONESIAN ENVIRONMENT AND ITS BIOSURFACTANT PRODUCTION ABILITY Bioemulsifier production, the biodegradation abilities and initial dioxygenase gene of a lessThan i greaterThan Bacillus lessThan /i greaterThan strain isolated from marine Indonesia was investigated. The 16S rDNA sequence revealed that the strain was lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan . The lessThan i greaterThan B. subtilis lessThan /i greaterThan possessed lessThan i greaterThan nahAc lessThan /i greaterThan and lessThan i greaterThan nidA lessThan /i greaterThan genes encoding the initial dioxygenase required for pyrene degradation. The lessThan i greaterThan B. subtilis lessThan /i greaterThan also could completely degrade a polycyclic aromatic hydrocarbon (PAH) of pyrene in 30 days. We suggest that the bioemulsifier that was also produced by the lessThan i greaterThan B. subtilis lessThan /i greaterThan , may assist the bacteria to attach the pyrene for degradation. HANIF YULIANI, HERI HERMANSYAH, MUHAMAD SAHLAN AND ANONDHO WIJANARKO Bioemulsifier, Bacillus subtilis, pyrene, biodegradation, dioxygenase 380-390