10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 4 2012(October - December) Multiple drug resistance of pathogenic bacteria is threat to human health and hence there is an urgent need to identify new drug targets. Due to advances in sequencing techniques complete genomic sequences and metabolic pathways of many bacterias are available. Comparative pathway analysis in pathogenic bacterias and Homo sapiens can lead to identification of new drug targets. In order to identify potential drug targets in lessThan i greaterThan Heamophilus influenzae lessThan /i greaterThan and lessThan i greaterThan Helicobacter Pylori lessThan /i greaterThan in silico metabolic pathways analysis was carried out. Metabolic enzymes of lessThan i greaterThan Heamophilus influenzae lessThan /i greaterThan and lessThan i greaterThan Helicobacter Pylori lessThan /i greaterThan with no homolog in lessThan i greaterThan Homo sapiens lessThan /i greaterThan have been identified at E value 0.01 by BLASTP. Essential enzymes and choke point reactions were identified by using database of essential genes and Choke point reaction finder tool respectively. Metabolic enzyme which are essential to pathogen, show no homolog in lessThan i greaterThan Homo sapiens lessThan /i greaterThan and catalyze choke point reactions have been identified as potential drug targets in current study. SNEHAL NANDODE, MANOJ DAMALE AND SANJAY HARKE Haemophilus influenza, Helicobacter Pylori, Metabolic enzyme analysis, Choke point reactions, Drug targets,Database of essential genes. 412-420