10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 4 2012(October - December) Antimicrobial activity of actinomycetes isolated from the soil was studied for the production of novel secondary metabolites. A total of 93 isolated actinomycetes were subjected to primary screening against human pathogenic lessThan i greaterThan Escherichia coli lessThan /i greaterThan , lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan , lessThan i greaterThan Candida albicans lessThan /i greaterThan and lessThan i greaterThan Aspergillus niger lessThan /i greaterThan . Out of 93 actinomycetes, only 25 isolates showed the antagonistic activity against the test pathogens. Six isolates were effective against lessThan i greaterThan Escherichia coli lessThan /i greaterThan , 10 were effective against lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan , 6 were effective against lessThan i greaterThan Candida albicans lessThan /i greaterThan and 19 were effective against lessThan i greaterThan Aspergillus niger lessThan /i greaterThan . Among them four isolates were effective against all the test pathogens. These four isolates were subjected to the secondary screening to test the broader spectrum activity. These isolates were identified by morphological and biochemical methods, 3 isolates were identified as lessThan i greaterThan Streptomyces lessThan /i greaterThan spp. and one isolate as lessThan i greaterThan Micromonospora lessThan /i greaterThan sp. DINESH PANWAR AND VIPIN K. SAINI Actinomycetes, Streptomyces, Agar well diffusion, Antagonistic activity, Broad spectrum antibiotics 500-506