10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 4 2012(October - December) The crown of thorns starfish (COT) lessThan i greaterThan Acanthaster planci lessThan /i greaterThan spine venom contains phospholipase A2 (PLA lessThan sub greaterThan 2 lessThan /sub greaterThan ). Recently, we have reported the rapid and efficient purification methods of it, especially for the PLA lessThan sub greaterThan 2 lessThan /sub greaterThan from COT spine isolated in Ambon islands. The COT isolated in Papua islands has a different morphology compared with COT in Ambon islands, the spines were bigger and longer. To confirm the availability of PLA lessThan sub greaterThan 2 lessThan /sub greaterThan in COT isolated in Papua, we isolated it by using our method. The activity of crude PLA lessThan sub greaterThan 2 lessThan /sub greaterThan of it higher compare with crude PLA lessThan sub greaterThan 2 lessThan /sub greaterThan isolated from COT spines from Ambon islands. Unfortunately, fractionated of ammonium sulfate at a concentration 20% is only slightly increased three times compared with the crude PLA lessThan sub greaterThan 2 lessThan /sub greaterThan . The result shows that the PLA lessThan sub greaterThan 2 lessThan /sub greaterThan is calcium-dependent enzyme. IMELDA KRISANTA ENDA SAVITRI, MUHAMADSAHLAN,FERA IBRAHIM AND ANONDHO WIJANARKO Crown of Thorns Starfish, Phospholipase A2, Calcium-dependent, purification. 603-608