10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 4 2012(October - December) This study aimed to describe an aerobic, gram positive, mesophilic and heterotrophic bacterium, designated OU35 isolated from polluted pond. In shake flask experiments, this bacterium produced a novel scl co mcl PHA, composed of 3-hydroxy butyrate (95 %), 3-hydroxy octanoate (2.5%), and 3-hydroxy decanoate (2.5%), from glucose and rice bran as carbon sources. The yield of the PHA was 1.217g/l lessThan sup greaterThan lessThan /sup greaterThan and1.530 g/l respectively. lessThan i greaterThan lessThan /i greaterThan Optimal growth occured between 28 and 37 lessThan sup greaterThan o lessThan /sup greaterThan C, at a PH between 6.0 and 7.8 and at an ionic strength of 12gl lessThan sup greaterThan -1 lessThan /sup greaterThan of sea salts. The G+C content of DNA is 45.25%. lessThan sup greaterThan lessThan /sup greaterThan Major fatty acids were iso-C lessThan sub greaterThan 15, 0 lessThan /sub greaterThan (20.29%), anteiso-C lessThan sub greaterThan 15, 0 lessThan /sub greaterThan (5.31%), iso-C lessThan sub greaterThan 16, 0 lessThan /sub greaterThan (8.35%), anteiso-C lessThan sub greaterThan 15,0 lessThan /sub greaterThan (5.31%), iso-C lessThan sub greaterThan 16,0 lessThan /sub greaterThan (8.35%). Strain exhibited maximum homology of 96.28% to lessThan i greaterThan Bacillus aryabhattai lessThan /i greaterThan B8W22 lessThan sup greaterThan T lessThan /sup greaterThan , lessThan i greaterThan lessThan /i greaterThan 95.97% to lessThan i greaterThan B.megaterium lessThan /i greaterThan IAM 13418 lessThan sup greaterThan T lessThan /sup greaterThan , and 95.282% to lessThan i greaterThan B. flexus lessThan /i greaterThan IFO15715 lessThan sup greaterThan T lessThan /sup greaterThan by 16S rDNA sequencing. The 16S rRNA gene sequence is deposited in EMBL/Genbank with accession number FN663629 and has been deposited in the CCTCC as strain CCTCC AB2011468 lessThan sup greaterThan T lessThan /sup greaterThan P.NAGAMANI, SK.MAHMOOD Bacillus, scl co mcl, PHA, Polyhydroxyakanoates 695-706