10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 4 2012(October - December) Yeast oils have several biotechnological applications such as fatty acid bioconversion, substrate valorization, polyunsaturated fatty acids production, oil polluted water bioremediation, biodiesel etc. Effects of physical and chemical parameters on lipid content in yeasts, therefore, deserve special consideration. In view of this, two oleaginous yeasts viz. lessThan i greaterThan Yarrowia lipolytica lessThan /i greaterThan (NCIM 3589) and lessThan i greaterThan Lipomyces lipofera lessThan /i greaterThan (NCIM 3252) were studied for their lipid producing capacities. We observed 1.6 times increase in lipids (8.0 %) in medium A1 for lessThan i greaterThan Y. lipolytica lessThan /i greaterThan and 1.9 times increase in lipids (2.4 %) in medium A2 for lessThan i greaterThan L. lipofera lessThan /i greaterThan as compared to MGYP lessThan i greaterThan . lessThan /i greaterThan In order to make the medium cost effective, glucose and peptone in MGYP were replaced with local commercial table sugar (LCTS) and urea respectively. Thereby 1.7 fold increase using medium B1 for lessThan i greaterThan Y. lipolytica lessThan /i greaterThan and 2.0 fold increase using medium B2 for lessThan i greaterThan L. lipofera lessThan /i greaterThan as compared to MGYP was achieved. In media B1 and B2, increase in fatty acids (monounsaturated and linoleic acid in both the yeasts and saturated in lessThan i greaterThan L. lipofera lessThan /i greaterThan ) was observed as compared to MGYP. VIPRA VIJAY JADHAV, DIPTI SAMBHAJI SALUNKHE AND RAMA KAUSTUBH BHADEKAR Cost effective media, Fatty acids, Lipids, Lipomyces lipofera, MGYP, Yarrowia lipolytica 757-769