10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 4 2012(October - December) Phenol tolerance and degradation using spent mycelium substrate of lessThan i greaterThan Hypsizygus ulmarius lessThan /i greaterThan showed that ligninolytic media with carbon or nitrogen limitation gave highest growth (26.27sq.cm), while limitation of both nutrients reduced it to 8.1sq.cm. 7.68sq.cm was seen in non-ligninolytic media; simultaneous nutrient increase gave 1.16sq.cm. Tolerance to phenol was best in ligninolytic media with carbon or nitrogen limitation. When phenol was introduced, growth decreased. Phenol increase from 200 to 400mg/L caused further decrease in nitrogen-optimum(7.03 to 2.35 sq.cm) and deficient(6.33 to 1.23 sq.cm) media, while nitrogen-deprived (2.48 to 5.52 sq.cm) media showed increase. Tolerance to higher phenol concentrations was accentuated by severe nitrogen limitation. Correlation of 0.99 between ligninolytic and non-ligninolytic media showed growth pattern similar irrespective of nutrient levels. Nitrogen reduction achieved 63% degradation from 200-800mg/L phenol, while carbon limitation gave 55.75%. Negligible degradation in non-ligninolytic condition. At lower phenol concentration, nitrogen limitation beneficial; for higher concentrations, carbon limitation helped. R. RANJINI AND T. PADMAVATHI Hypsizygus ulmarius, Ligninolytic media, Non-ligninolytic media, Phenol Tolerance and Degradation Profile, Spent Mycelium Substrate. 987-994