10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 4 2012(October - December) Imidacloprid (I-[(6-chloro-3-pyridinyl)-methyl]-N-nitro-2-imidazolidinimine), a chloronicotinyl insecticide is used widely to control biting and sucking insects. Present investigation was carried out to analyse the effect of imidacloprid on biochemical parameters like DNA, RNA, protein and glucose in soil isolate lessThan i greaterThan Escherichia coli lessThan /i greaterThan , further the toxic effect of the insecticide on the growth was also studied. The study involving soil isolate lessThan i greaterThan Escherichia coli lessThan /i greaterThan with molar concentrations of 10 lessThan sup greaterThan -3 lessThan /sup greaterThan to 10 lessThan sup greaterThan -7 lessThan /sup greaterThan of imidacloprid insecticide showed that there was an increase in the percent inhibition of DNA, RNA, protein and glucose, the inhibitory effect increased with an increase in the concentration of insecticide proving that the inhibitory effect is dose dependent. Further, imidacloprid inhibited the growth of lessThan i greaterThan Escherichia coli lessThan /i greaterThan when compared to control. The present investigation indicates that imidacloprid reduced the DNA RNA, glucose and protein content which intern effects the growth of the lessThan i greaterThan Escherichia coli lessThan /i greaterThan . A. A SHETTI, A. G. KULKARNI, R. B. KALIWAL, C. T. SHIVASHARANA AND B. B. KALIWAL Imidacloprid, Escherichia coli, Biochemical Parameters, Growth 1155-1163