10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 4 2012(October - December) The green synthesis of silver nanoparticles is a basic need in the field of nanotechnology. In present study, the silver nanoparticles were synthesized using lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan supernatant alone and lessThan i greaterThan Bacillus subtilis lessThan /i greaterThan supernatant in presence of glucose. UV-visible spectrophotometer analysis was carried out to assess the synthesis of silver nanoparticles. The synthesized silver nanoparticles were further characterized by using Nanoparticle Tracking Analyzer (NTA), Transmission Electron Microscope (TEM) and Energy Dispersive X-ray Spectra (EDX). Silver nanoparticles with controlled size and shape were observed under TEM micrograph. These silver nanoparticles showed enhanced quorum quenching activity against lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan biofilm which was observed under inverted microscope. In near future, silver nanoparticles synthesized using green methods may be used in treatment of infections caused by highly antibiotic resistant biofilm. SHALAKA A. MASURKAR , PRATIK R. CHAUDHARI AND SURESH P. KAMBLE Bacillus subtilis; Biofilm; Quorum quenching; Silver nanoparticles 48-54