10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 4 2012(October - December) A novel isocratic reverse-phase high performance liquid-chromatography method for determination of asenapine maleate was developed and validated after optimization of various chromatographic conditions. Samples were separated on a waters x-terra C lessThan sub greaterThan 18 lessThan /sub greaterThan (100 mm × 4.6 mm, 3.5 μ) analytical column. The mobile phase used was acetonitrile: 0.1M phosphate buffer (p lessThan sup greaterThan H lessThan /sup greaterThan 3.2) 65:35%v/v operated at 30 °C column oven temperature was pumped at a flow rate of 1.0 mL min lessThan sup greaterThan −1 lessThan /sup greaterThan and the column eluents were monitored at a wavelength of 272 nm. When sample was injected into the Finnigan surveyor high performance liquid-chromatography system through Finnigan surveyor auto-sampler injector, separation was achieved within 5.0 min. The present method was demonstrated and it was validated with the acceptable values for selectivity, linearity (within the expected concentration range (10–50 μg mL lessThan sup greaterThan −1 lessThan /sup greaterThan ; lessThan i greaterThan r lessThan /i greaterThan lessThan sup greaterThan 2 lessThan /sup greaterThan   greaterThan  0.999)), recovery ( greaterThan 95%), precision (%RSD  T.R.PARTHASARATHI, TAMIL SELVI SRINIVAS, M.VANITHA SRI, SEGU SAI RAM, MULLA MAHABOOB BASHA AND PUDI RAJESH. Asenapine maleate, pharmaceutical dosage form, RP-HPLC, Validation. 360-366