10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 4 2012(October - December) The present study investigated the inhibitory effect of methanolic extract of lessThan i greaterThan Couroupita guianensis lessThan /i greaterThan leaves (MCG) on carbohydrate hydrolysing enzymes and glucose uptake in isolated rat hemidiaphragm. Method: The effects of MCG at different concentrations (1.25-10mg) were carried out on carbohydrate hydrolysing enzymes namely porcine pancreatic α- amylase, rat intestinal α-glucosidase and sucrase. The effect of MCG on glucose utilisation was evaluated by estimating glucose uptake in isolated rat hemidiaphragm. Results: MCG showed significant dose dependent on inhibition of rat intestinal sucrase (IC lessThan sub greaterThan 50 lessThan /sub greaterThan  = 2.38 mg/ml), rat intestinal maltase (IC lessThan sub greaterThan 50 lessThan /sub greaterThan  = 2.5mg/ml) and porcine pancreatic α-amylase. (IC lessThan sub greaterThan 50 lessThan /sub greaterThan  = 2.86 mg/ml). Furthermore, the glucose uptake in isolated rat hemidiaphragm was increased (P lessThan 0.001) by MCG when compared to the control group. Conclusion: These results suggest that MCG has significant inhibitory activity on carbohydrate metabolizing enzymes and increases the peripheral utilization of glucose, indicating its probable therapeutic use as a potent antihyperglycemic agent. GAURESH SOMANI, RAHUL CHAUDHARI, JAYANT SANCHETI,AND SADHANA SATHAYE α-Glucosidase, α-Amylase, Couroupita guianensis leaves 511-520