10.22376/ijpbs.2019.10.1.p1-12 Volume 3 Issue 4 2012(October - December) The antimicrobial activity of silver nanoparticles with the clinically important microbes is a major area of research in the field of nanobiotechnology. lessThan i greaterThan Syzgium cumini lessThan /i greaterThan (L.) is used as medicinal plant for anti-diabetic property. The leaves and bark are used for controlling blood pressure and gingivitis. In this present study, we have synthesized silver nanoparticles using lessThan i greaterThan Syzygium cumini lessThan /i greaterThan leaf extract. The leaf extract of lessThan i greaterThan S. cumini lessThan /i greaterThan is used as capping and reducing agent in the synthesis of silver nanoparticles. The formation of nanoparticles was confirmed by UV-Vis spectrophotometer, Atomic Force Microscopy (AFM) and Scanning Electron Microscopy (SEM). Further antimicrobial activity of silver nanoparticles was studied against lessThan i greaterThan Escherichia coli lessThan /i greaterThan MTCC 1302, lessThan i greaterThan Staphylococcus aureus lessThan /i greaterThan MTCC 740 lessThan i greaterThan , Pseudomonas aeruginosa lessThan /i greaterThan MTCC 2295 lessThan i greaterThan , and Bacillus licheniformis lessThan /i greaterThan MTCC 9555. Surface Plasmon resonance of silver nanoparticles was recorded at 400 nm by UV-Vis spectrophotometer. The synthesized nanoparticles are spherical and having size ranging from 100- 160 nm. RAM PRASAD,V. SATYANARAYANA SWAMY ,KUMAR SURANJIT PRASAD AND AJIT VARMA Syzygium cumini, Silver nanoparticles, Antimicrobial activity, Atomic force microscopy, Scanning electron microscopy, leaf extract 745-752