10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 1 2013 (January - March) Aim of the present study was to evaluate the awareness of health workers about universal work precautions and implementation of their knowledge in private institutes of Latur. Methodology Cross-sectional study of health workers was conducted using a self-administered questionnaire, which enquired about knowledge, attitude and practices of universal work precautions. Results: 210 questionnaires were sent to health workers and 150 of them were returned giving a response rate of 72%. 96% of the participants were aware of universal work precautions. Out of 150 participants 53.33% were injured while working and 68.75% of them used the first aid box. All the participants wore gloves during laboratory work but 53.43 % wore a single pair. 47.94 % of the participants claimed to know what to do if exposed to infection. 5.33% of the participants ate in the laboratory, 2.22% of them stored foods and water in the refrigerators, 2.22% of them put on cosmetics in the laboratory. 61.33% were immunized against hepatitis B virus (HBV). 47.94% of them did take shower immediately after laboratory work. 58.0% of the participants did not feel that the use of masks is necessary at the place of work. Conclusion: The knowledge, attitude and practical implementation of universal work precautions amongst health workers is poor. DR.R.H.DESHPANDE AND DR.S.K.WADDE Universal Work Precautions, Health workers, knowledge, attitude and Practices. 163-168