10.22376/ijpbs.2019.10.1.p1-12 Volume 4 Issue 1 2013 (January - March) Lactation was induced in four non-pregnant, non-lactating buffaloes by subcutaneous injections of estradiol-17β and progesterone for 10 d @ 0.10 and 0.25 mg/kg b. w. /d respectively and Dexamethasone (@ 0.028 mg/kg b.w./d) treatment was given on 17 lessThan sup greaterThan th lessThan /sup greaterThan to 19 lessThan sup greaterThan th lessThan /sup greaterThan d. Milking was initiated on day 20. Biopsies of mammary glands were collected on 0, 7 lessThan sup greaterThan th lessThan /sup greaterThan , 14 lessThan sup greaterThan th lessThan /sup greaterThan , and 21 lessThan sup greaterThan st lessThan /sup greaterThan day from each animal. On 0 lessThan sup greaterThan th lessThan /sup greaterThan day of hormonal treatment on mammary tissues there was abundant connective and adipose tissues with very sparse lobuloalveolar structures. On 7 lessThan sup greaterThan th lessThan /sup greaterThan day, there was decrease in stroma, increase in epithelial cells area with increased lobulo-alveolar architecture. There was accumulation of intra-cellular and intra-luminal secretions with more lipid droplets. From 7 lessThan sup greaterThan th lessThan /sup greaterThan to 21 lessThan sup greaterThan st lessThan /sup greaterThan day, these changes were progressive although variable amongst buffaloes. The average size of the lobule, alveoli as well as number and volume of alveoli were significantly increased on the 21 lessThan sup greaterThan st lessThan /sup greaterThan day as compared to 0 day. H.H. MEHTA, A.K. PATEL, K.N. NANDASANA, U.V. RAMANI, P.G. KORINGA, R.G. SHAH, D. R. BARVALIA, N.H. KELAWALA, D.N. RANK, C.G.JOSHI, K.M. PANCHAL AND R.K. KOTHARI Induction, Progesterone, Estrogen, Mammary Gland, Alveoli, Lobules 298-308